Scale-Free Topology of the CA3 Hippocampal Network: A Novel Method to Analyze Functional Neuronal Assemblies
Scale-Free Topology of the CA3 Hippocampal Network: A Novel Method to Analyze Functional Neuronal Assemblies
复制标题
CA3 海马网络的无标度拓扑：一种分析功能神经元组件的新方法
DOI：
10.1016/j.bpj.2010.01.013
复制
发表时间：
2010-05-05
影响因子：
3.4
通讯作者：
Sik, Attila
中科院分区：
文献类型：
--
作者：
Li, Xiaoli;Ouyang, Gaoxiang;Sik, Attila
海马的认知映射功能主要依赖于CA 3锥体细胞的递归网络。然而，人们仍然不知道网络活动模式是如何出现的，或者它们是如何编码信息的。应用功能性多神经元钙离子成像技术，我们同时记录了海马脑片培养的CA 3区100多个神经元的活动。我们利用一种新的计算方法来分析多通道的棘波序列，并描绘功能的神经元组装。利用事件同步和相关矩阵分析方法，我们发现：1）平均功能神经元簇由23个神经元组成，神经元可以是多个集合体的一部分：2）神经元集合体的簇强度、簇大小和细胞间平均距离均服从幂律分布;（3）神经元聚集体的聚集强度和大小与神经元总数和它们之间的物理距离无关; 4）神经元聚集体的聚集距离与神经元总数和它们的物理距离弱相关。这些发现表明，自发放电的CA 3海马网络的功能组织是一个无标度的结构在切片培养。
Cognitive mapping functions of the hippocampus critically depend on the recurrent network of the CA3 pyramidal cells. However, it is still not known in detail how network activity patterns emerge, or how they encode information. By using functional multineuron calcium imaging, we simultaneously recorded the activity of >100 neurons in the CA3 region of hippocampal slice cultures. We utilized a novel computational method to analyze the multichannel spike trains and to depict functional neuronal assemblies. By means of event synchronization and the correlation matrix analysis method, we found that: 1), the average functional neuronal cluster consists of 23 neurons, and neurons could be part of multiple assemblies; 2), the clustering strength, size, and mean distance among cells in neuronal assemblies follow a power-law-like distribution; 3), the clustering strength and size of neuronal assemblies are not correlated with the total number of neurons and their physical distance; and 4), the clustering distance of neuronal assemblies is weakly correlated with the total number of neurons and their physical distance. These findings suggest that the functional organization of the spontaneously firing CA3 hippocampal network is a scale-free structure in slice culture.