The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis
The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis
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DOI：
10.1093/plcell/koac014
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发表时间：
2022-01-28
期刊：
影响因子：
11.6
通讯作者：
Sato, Takeo
中科院分区：
文献类型：
--
作者：
Hasegawa, Yoko;Reyes, Thais Huarancca;Sato, Takeo
TGN/EE SNARE蛋白SYP 61在植物碳/氮营养胁迫应答中起着关键作用，在低碳/高氮条件下被泛素化，泛素化是一种翻译后修饰，涉及小蛋白泛素与靶蛋白的可逆连接。泛素化参与许多细胞过程，包括货物蛋白的膜运输。然而，对贩运机制各组成部分的普遍存在及其参与环境对策的情况还没有很好的了解。在这里，我们报告了拟南芥trans-Golgi网络/早期内体定位SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）蛋白SYP 61与跨膜泛素连接酶ATL 31相互作用，ATL 31是对破坏的碳（C）/氮/（N）营养条件的抗性的关键调节因子。SYP 61是拟南芥细胞膜运输的关键组分。SYP 61敲低突变体中ATL 31的亚细胞定位被破坏，并且ATL 31过表达植物对高C/低N胁迫的不敏感性在这些突变体中被抑制，这表明SYP 61和ATL 31在植物对营养胁迫的响应中协同起作用。SYP 61在植物中被泛素化，并且其泛素化水平在低C/高N营养条件下上调。这些发现提供了重要的见解泛素信号和膜运输机制在植物中。
The TGN/EE SNARE protein SYP61 plays a critical role in plant carbon/nitrogen nutrient stress responses and is ubiquitinated in response to low carbon/high nitrogen conditions.Ubiquitination is a post-translational modification involving the reversible attachment of the small protein ubiquitin to a target protein. Ubiquitination is involved in numerous cellular processes, including the membrane trafficking of cargo proteins. However, the ubiquitination of the trafficking machinery components and their involvement in environmental responses are not well understood. Here, we report that the Arabidopsis thalianatrans-Golgi network/early endosome localized SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein SYP61 interacts with the transmembrane ubiquitin ligase ATL31, a key regulator of resistance to disrupted carbon (C)/nitrogen/(N)-nutrient conditions. SYP61 is a key component of membrane trafficking in Arabidopsis. The subcellular localization of ATL31 was disrupted in knockdown mutants of SYP61, and the insensitivity of ATL31-overexpressing plants to high C/low N-stress was repressed in these mutants, suggesting that SYP61 and ATL31 cooperatively function in plant responses to nutrient stress. SYP61 is ubiquitinated in plants, and its ubiquitination level is upregulated under low C/high N-nutrient conditions. These findings provide important insights into the ubiquitin signaling and membrane trafficking machinery in plants.