Enhanced survival and regeneration of axotomized retinal ganglion cells by a mixture of herbal extracts
Enhanced survival and regeneration of axotomized retinal ganglion cells by a mixture of herbal extracts
复制标题
DOI：
10.1089/089771502753594936
复制
发表时间：
2002-03-01
影响因子：
4.2
通讯作者：
Chen, CF
中科院分区：
文献类型：
--
作者：
Cheung, ZH;So, KF;Chen, CF
本研究旨在探讨西番参提取物（PQE）、银杏叶提取物（GBE）和贯叶连翘提取物（HPE）联合使用或单独使用对成年仓鼠视神经横断模型视网膜神经节细胞（RGCs）存活和再生的影响。单侧视神经横断，以评估草药提取物对axotomy RGCs存活的影响。另一方面，通过将周围神经移植到截断的眼残端诱导再生，研究了草药提取物对轴突再生的影响。在存活和再生实验中，手术动物每天口服给药或草药提取物（PQE、GBE和HPE），单独或联合给药，分别持续7天和21天。用氟金逆行标记存活和再生的RGCs。然后将眼睛去核，将视网膜平装以计数标记的rgc。单独使用PQE、GBE和HPE治疗不能对损伤的RGCs提供神经保护。然而，使用Menta-FX （PQE、GBE和HPE的混合物）治疗，可显著提高直肠切开术后7天的RGC存活率。使用Menta-FX治疗视神经横断21天后，再生RGCs的数量也显著增加（87%）。本研究表明，草药可作为受损rgc的潜在神经保护剂。它还表明，草药疗法的治疗价值可以通过使用适当的草药混合物来最大化。
The aim of this study is to investigate the effects of Panax quinquefolius L. extract (PQE), Ginkgo biloba extract (GBE), and Hypericum perforatum extract (HPE), in combination or alone, on the survival and regeneration of axotomized retinal ganglion cells (RGCs) in an optic nerve transection model in adult hamsters. Unilateral transection of the optic nerve was performed to evaluate the effects of herbal extracts on the survival of axotomized RGCs. Effects of the herbal extracts on axonal regeneration of axotomized RGCs, on the other hand, were studied by attaching a peripheral nerve graft onto the transected ocular stump to induce regeneration. Operated animals received daily oral administration of vehicle or herbal extracts (PQE, GBE, and HPE), alone or in combination, for 7 and 21 days, respectively, in the survival and regeneration experiments. Surviving and regenerating RGCs were retrogradely labeled with Fluoro-Gold. The eyes were then enucleated and the retinas were flat-mounted for the counting of the labeled RGCs. Treatment with PQE, GBE and HPE alone failed to offer neuroprotection to injured RGCs. However, treatment with Menta-FX, a mixture of PQE, GBE, and HPE, significantly augmented RGC survival 7 days postaxotomy. Treatment with Menta-FX also induced a significant (87%) increase in the number of regenerating RGCs 21 days after optic nerve transection. This study demonstrates that herbs can act as a potential neuroprotective agent for damaged RGCs. It also suggests that the therapeutic value of herbal remedies can be maximized by the use of mixtures of appropriate herbs.