Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C280 and EspB proteins
Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C280 and EspB proteins
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DOI：
10.1016/j.vaccine.2011.03.079
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发表时间：
2011-05-23
期刊：
影响因子：
5.5
通讯作者：
Mercado, Elsa C.
中科院分区：
文献类型：
--
作者：
Vilte, Daniel A.;Larzabal, Mariano;Mercado, Elsa C.
肠出血性大肠杆菌(EHEC)O157：H7是全世界从溶血性尿毒症综合征(HUS)患者中恢复的最流行的EHEC血清型。为牛接种疫苗可能是对抗人类感染的合理策略。牛是EHEC O157：H7的主要宿主。本研究以O157：H7的两个关键定植因子--γ-内膜蛋白C-280和EspB的280个氨基酸的羧基末端片段为基础，对疫苗进行了评价。肌肉免疫可显著提高血清中抗这两种蛋白的抗体水平。唾液中也可诱导出抗原特异性的IgA和Ig G，但仅有IgA反应显著。在对大肠杆菌O157：H7进行实验攻击后，与对照组相比，接种疫苗的小牛的细菌脱落显著减少。这些有希望的结果表明，用INININ和EspB对牛进行系统免疫可能是减少EHEC O157：H7粪便脱落的可行策略。(C)2011爱思唯尔有限公司。保留所有权利。
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of gamma-intimin (gamma-intimin C-280) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle. (C) 2011 Elsevier Ltd. All rights reserved.