Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway
Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway
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DOI：
10.7554/elife.40444
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发表时间：
2018-10-19
期刊：
影响因子：
7.7
通讯作者：
Chaput, John C.
中科院分区：
文献类型：
--
作者：
Chim, Nicholas;Jackson, Lynnette N.;Chaput, John C.
DNA聚合酶中间体的高分辨率晶体结构是研究细胞内DNA合成机制的必要条件。在这里，我们报告了DNA聚合酶I的五种晶体结构，它们捕获了DNA合成途径中聚合酶易位和核苷酸预插入步骤的新构象。我们认为，这些新结构，以及以前解决的结构，突出了酶活性位点的手指亚结构域的动态性质。
High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.