Remote Control of Lipophilic Nucleic Acids Domain Partitioning by DNA Hybridization and Enzymatic Cleavage
Remote Control of Lipophilic Nucleic Acids Domain Partitioning by DNA Hybridization and Enzymatic Cleavage
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DOI：
10.1021/ja309256t
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发表时间：
2012-12-19
影响因子：
15
通讯作者：
Arbuzova, Anna
中科院分区：
文献类型：
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作者：
Schade, Matthias;Knoll, Andrea;Arbuzova, Anna
液体无序（Id）和液体有序（lo）结构域之间的脂质修饰分子的横向分区取决于脂质修饰的类型，间隔物的存在，膜组成和温度。在这里，我们表明，棕榈酰化肽核酸（PNA）的LO域分区可以通过形成一个四组分复合物的Id域分区生育酚修饰的DNA的影响：PNA DNA复合物分配到Id域。DNA接头的酶促裂解导致复合物的破坏，并恢复了亲脂性核酸到相应结构域中的初始分布。该模块化系统提供了仿生表面的动态功能化策略，例如，在纳米结构化和酶催化的调节中，并且它提供了一种工具来研究膜中脂质修饰蛋白质的受控重组的分子基础，例如，在信号转导期间。
Lateral partitioning of lipid-modified molecules between liquid-disordered (Id) and liquid-ordered (lo) domains depends on the type of lipid modification, presence of a spacer, membrane composition, and temperature. Here, we show that the lo domain partitioning of the palmitoylated peptide nucleic acid (PNA) can be influenced by formation of a four-component complex with the Id domain partitioning tocopherol-modified DNA: the PNA DNA complex partitioned into the Id domains. Enzymatic cleavage of the DNA linker led to the disruption of the complex and restored the initial distribution of the lipophilic nucleic acids into the respective domains. This modular system offers strategies for dynamic functionalization of biomimetic surfaces, for example, in nanostructuring and regulation of enzyme catalysis, and it provides a tool to study the molecular basis of controlled reorganization of lipid-modified proteins in membranes, for example, during signal transduction.