Antibody array-based proteomic screening of novel biomarkers in malignant biliary stricture.
Antibody array-based proteomic screening of novel biomarkers in malignant biliary stricture.
复制标题
基于抗体阵列的蛋白质组学筛选恶性胆管狭窄中的新型生物标志物。
DOI：
10.3233/cbm-210095
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bing Hu
中科院分区：
文献类型：
--
作者：
Wen;Jun;Shu;Zhi;Kunke Wang;Bing Hu
背景
良恶性胆管狭窄的鉴别诊断一直是临床实践中的一个挑战。
目的
本研究旨在发现胆汁中新的生物标志物，以提高恶性胆道狭窄的诊断准确性。
方法
分别收集6例恶性和良性胆管狭窄患者的胆汁样品。用440种蛋白质的半定量人抗体阵列分析胆汁的蛋白质谱。通过Venn图分析筛选差异表达蛋白。此后，使用lasso分析在较大（n= 40）患者组中验证了这些潜在生物标志物用于区分恶性和非恶性胆管狭窄的准确性。
结果
发现20种蛋白质在恶性与良性胆管狭窄中差异表达，其中6种通过Venn图分析鉴定为上调，无论胆管狭窄的位置如何。在6种生物标志物中，胆汁脂质运载蛋白-2、P-钙粘蛋白和脂蛋白酶显示出比胆汁CA 19 -9更好的诊断实用性。Lasso分析确定，脂质运载蛋白-2、P-钙粘蛋白和CA 19 -9作为一组标记物最能区分恶性狭窄和良性狭窄。
结论
胆汁中Lipocalin-2和P-cadherin的测定可用于临床恶性胆管狭窄的检测。
BACKGROUND
Distinguishing between benign and malignant bile duct strictures has long been a diagnostic challenge in clinical practice.
OBJECTIVE
This study aimed to discover novel biomarkers in bile to improve the diagnostic accuracy of malignant biliary strictures.
METHODS
Bile samples were collected from 6 patients with malignant or benign biliary stricture, respectively. Protein profiles of the bile were analyzed with a semi-quantitative human antibody array of 440 proteins. Then the differential expressed proteins were screened by Venn diagram analysis. Following this, the accuracy of these potential biomarkers for discriminating between malignant and non-malignant biliary strictures was validated in a larger (n= 40) group of patients using lasso analysis.
RESULTS
Twenty proteins were found differentially expressed in malignant versus benign biliary strictures, 6 of which were identified by Venn diagram analysis to be up-regulated regardless of the location of biliary strictures. Among the 6 biomarkers, bile lipocalin-2, P-cadherin, and adipsin showed better diagnostic utility than that of bile CA19-9. Lasso analysis identified that lipocalin-2, P-cadherin and CA19-9 as a group of makers best distinguished malignant from benign strictures.
CONCLUSIONS
Lipocalin-2 and P-cadherin measurements in bile could be clinically useful for the detection of malignant biliary strictures.
影响因子：
8
作者：
Goto, Hideaki;Shimono, Yohei;Minami, Hironobu
通讯作者：
Minami, Hironobu