B7-H4 overexpression in ovarian tumors
B7-H4 overexpression in ovarian tumors
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DOI：
10.1016/j.ygyno.2005.08.060
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发表时间：
2006-01-01
影响因子：
4.7
通讯作者：
Shroyer, KR
中科院分区：
文献类型：
--
作者：
Tringler, B;Liu, WH;Shroyer, KR
目标.尽管在治疗管理方面取得了很大进展，但在过去的50年里，卵巢癌的死亡率仍然相对稳定。本研究旨在评估B7-H4蛋白在良性肿瘤、低度恶性肿瘤和卵巢恶性肿瘤中的表达。通过免疫组织化学方法评价来自浆液性、粘液性、类胶质瘤和透明细胞卵巢肿瘤的福尔马林固定的存档组织块中B7-H4表达的分布，并通过自动图像分析测量染色强度。单变量分析用于检验统计学显著性关系。B7-H4在原发性浆液性卵巢癌（n = 32）和类浆液性卵巢癌（n = 12）中均呈胞浆和胞膜表达。透明细胞癌15例，转移性浆液性癌23例，类浆液性癌7例。相比之下，局灶性B7-H4表达中检测到只有1/11粘液癌。浆液性癌的阳性细胞比例和染色强度中位数均高于浆液性囊腺瘤和低度恶性浆液性肿瘤，差异有统计学意义（分别为P < 0.0001和P = 0.034）。子宫内膜样癌的染色强度中位数也显著高于子宫内膜异位症（P = 0.005）。B7-H4在浆液性、类浆液性和透明细胞卵巢癌中的一致性过表达和在大多数正常体细胞组织中的相对缺乏表达表明B7-H4应进一步研究作为卵巢癌的潜在诊断标志物或治疗靶点。(c)2005年爱思唯尔公司All rights reserved.
Objectives. Despite great advances in therapeutic management, the mortality rate for ovarian cancer has remained relatively stable over the past 50 years. This study was designed to evaluate the expression of B7-H4 protein, recently identified as a potential molecular marker of breast and ovarian cancer by quantitative PCR analysis, in benign tumors, tumors of low malignant potential and malignant tumors of the ovary.Methods. Archival formalin-fixed tissue blocks from serous, mucinous, endometrioid and clear cell ovarian tumors were evaluated by immunohistochemistry for the distribution of B7-H4 expression, and staining intensity was measured by automated image analysis. Univariate analyses were used to test for statistically significant relationships.Results. B7-H4 cytoplasmic and membranous expression was detected in all primary serous (n = 32), endometrioid (it 12). and clear cell carcinomas (it = 15), and in all metastatic serous (it = 23) and endometrioid (it = 7) ovarian carcinomas. By contrast, focal B7-H4 expression was detected in only 1/11 mucinous carcinomas. The proportion of positive cells and median staining intensity was greater in serous carcinomas than in serous cystadenomas or serous tumors of low malignant potential, and the differences were statistically significant (P < 0.0001 and P = 0.034, respectively). The median staining intensity was also significantly greater in endometrioid carcinomas than in endometriosis (P = 0.005).Conclusions. The consistent overexpression of B7-H4 in serous, endometrioid and clear cell ovarian carcinomas and the relative absence of expression in most normal somatic tissues indicates that B7-H4 should be further investigated as a potential diagnostic marker or therapeutic target for ovarian cancer. (c) 2005 Elsevier Inc. All rights reserved.