HIGH-RESOLUTION ELECTRON CRYSTALLOGRAPHY OF PROTEIN MOLECULES
HIGH-RESOLUTION ELECTRON CRYSTALLOGRAPHY OF PROTEIN MOLECULES
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DOI：
10.1016/0304-3991(93)90064-5
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发表时间：
1993-12-01
期刊：
影响因子：
2.2
通讯作者：
DOWNING, KH
中科院分区：
文献类型：
--
作者：
GLAESER, RM;DOWNING, KH
现在可以使用电子衍射数据和高分辨率图像来获得生物大分子的精确三维密度图。这些密度图可以通过将结构的原子分辨率模型构建到实验密度中来解释。动态衍射理论的 Cowley-Moodie 形式已用于验证运动衍射理论（严格来说，弱相物体近似）在生成此类 3D 密度图时的使用。在非常平坦（平面）样品的制备以及将衍射保留至 0.2 nm 或更好的分辨率方面的进一步改进可能会导致电子晶体学成为与当前结构分子生物学领域的 X 射线晶体学一样重要的技术。
Electron diffraction data and high-resolution images can now be used to obtain accurate, three-dimensional density maps of biological macromolecules. These density maps can be interpreted by building an atomic-resolution model of the structure into the experimental density. The Cowley-Moodie formalism of dynamical diffraction theory has been used to validate the use of kinematic diffraction theory (strictly, the weak phase object approximation) in producing such 3D density maps. Further improvements in the preparation of very flat (planar) specimens and in the retention of diffraction to a resolution of 0.2 nm or better could result in electron crystallography becoming as important a technique as X-ray crystallography currently is for the field of structural molecular biology.