Autoradiographic localization of a binding protein(s) specific for prostaglandin D2 in rat brain.
Autoradiographic localization of a binding protein(s) specific for prostaglandin D2 in rat brain.
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大鼠脑中前列腺素 D2 特异性结合蛋白的放射自显影定位。
DOI：
10.1073/pnas.80.19.6114
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发表时间：
1983
影响因子：
11.1
通讯作者：
O. Hayaishi
中科院分区：
文献类型：
--
作者：
A. Yamashita;Y. Watanabe;O. Hayaishi
采用2%多聚甲醛灌注固定大鼠脑切片，检测[3H]前列腺素（PG） D2的特异性结合。这种结合是可逆的、可饱和的、高亲和力的、Na+依赖性的，并且对PGD2具有高度特异性。这些结合特性与先前报道的在大鼠脑突触膜上观察到的特征基本相似。采用计算机密度测定法和彩色编码技术，对大鼠脑内[3H]PGD2结合区域进行了图像分析。在大脑皮层、视前区、杏仁核、下丘脑核（弓状核、腹内侧核和下丘脑后核）、丘脑核（团聚核和菱形核）、海马、松果体和小脑皮质中观察到高密度的结合位点。纹状体中没有明显的结合，白质、蛛网膜和脉管系统中也没有。
The specific [3H]prostaglandin (PG) D2 binding was detected by using the slide-mounted sections of rat brain fixed by perfusion with 2% paraformaldehyde. The binding was reversible, saturable, high affinity, Na+ dependent, and highly specific for PGD2. These binding characteristics are essentially similar to those observed with the synaptic membrane of rat brain as previously reported. Using autoradiographic image analyses by computerized densitometry and color coding, we visualized the localization of [3H]PGD2 binding in rat brain. A high density of the binding sites was observed in the cerebral cortex, preoptic area, amygdala, hypothalamic nuclei (arcuate nucleus, ventromedial nucleus, and posterior hypothalamic nucleus), thalamic nuclei (reuniens nucleus and rhomboid nucleus), hippocampus, pineal body, and cerebellar cortex. The binding was not significantly observed in the striatum and also was negative in the white matter, arachnoid membranes, and vasculatures.