Image analysis of the extracellular matrix.
Image analysis of the extracellular matrix.
复制标题
细胞外基质的图像分析。
DOI：
10.1002/jemt.1070280507
复制
发表时间：
1994
影响因子：
2.5
通讯作者：
Kohler,DM
中科院分区：
文献类型：
--
作者：
Arsenault,AL;Kohler,DM
骨骺生长板和关节软骨基质通过急速冷冻和冷冻置换来保存，以最佳地保留细胞和基质组分的天然组织。这些标本进行染色，并使用传统的电子显微镜方法进行检查。使用分布、连接性、方向和各种形态测定分析等参数，通过计算机图像分析检查高度整合的富含蛋白聚糖的基质。此外，不同方面的电子断层扫描和3D渲染的基质囊泡及其相关的矿物沉积从骺生长板和火鸡腿肌腱。© 1994 Wiley利斯公司
The epiphyseal growth plate and articular cartilage matrices were preserved by slam freezing and freeze substitution to optimally retain the native organization for both cellular and matrix components. These specimens were stained and examined using conventional electron microscopic methods. The highly integrated, proteoglycan‐rich matrices were examined by computer image analysis using such parameters as distribution, connectivity, orientation, and a variety of morphometric analyses. Also, different aspects of electron tomography and 3D rendering of matrix vesicles and their associated mineral deposits from epiphyseal growth plates and turkey leg tendons are presented. © 1994 Wiley‐Liss, Inc.