A preliminary investigation of the effect of relaxin on bone remodelling in suture expansion
A preliminary investigation of the effect of relaxin on bone remodelling in suture expansion
复制标题
松弛素对缝线扩张骨重建影响的初步研究
DOI：
--
复制
发表时间：
2016
影响因子：
2.6
通讯作者：
K. Moriyama
中科院分区：
文献类型：
--
作者：
C. Duarte;Yukiho Kobayashi;Jumpei Morita;T. Kawamoto;K. Moriyama
背景和目标
松弛素（RLN）是一种与细胞外基质降解、破骨细胞生成和成骨细胞分化相关的胰岛素样激素。本研究旨在评估RLN在小鼠颅骨矢状缝侧向扩张期间和之后的作用。
材料和方法
局部注射RLN使用纳米尺寸的脂质体载体到矢状缝的8至10周龄的野生型小鼠就在横向扩张。通过显微计算机断层扫描分析缝线形态、骨矿物质密度（BMD）和骨体积。胶原沉积和破骨细胞分化分别通过Verhoeff-Van Gieson（VVG）和抗酒石酸酸性磷酸酶（TRAP）染色观察。
结果
48小时后，在RLN处理的样品中观察到较少的胶原染色和较高的组织特异性松弛素/胰岛素样家族肽受体（Rxfp）-1和-2表达。1周后，在RLN处理的样本中观察到BMD和体积增加，厚的组织良好的类骨质组织，多核TRAP阳性细胞。Rxfp-1的表达增加，观察到在矢状缝中的中缝纤维组织后RLN治疗。Rxfp-2仅在张力刺激下的颅骨中表达，并且RLN处理进一步增加其表达。
限制
在当前实验条件下，在任何情况下均未检测到RLN-脂质体。这是一项初步研究，样本数量限制了其结果的效力。VVG染色不能定量胶原含量，但可以提供胶原纤维存在的初步信息。
结论
RLN治疗可在缝线扩张期间和之后改变骨重塑和胶原代谢。
Background and objectives
Relaxin (RLN) is an insulin-like hormone associated with extracellular matrix degradation, osteoclastogenesis, and osteoblast differentiation. This study aimed to assess the effect of RLN during and after lateral expansion of murine calvarial sagittal sutures.
Materials and methods
RLN was injected topically using a nano-sized liposome carrier into the sagittal sutures of 8- to 10-week-old wild type mice just before lateral expansion. Suture morphology, bone mineral density (BMD), and bone volume were analysed by micro-computed tomography. Collagen deposition and osteoclast differentiation were observed by Verhoeff-Van Gieson (VVG) and tartrate-resistant acid phosphatase (TRAP) staining, respectively.
Results
Less collagen staining and higher tissue-specific relaxin/insulin-like family peptide receptor (Rxfp)-1 and -2 expression were observed in the RLN-treated samples after 48 hours. Increased BMD and volume, and thick well-organised osteoid tissue, with multinucleated TRAP-positive cells, were observed in RLN-treated samples after 1 week. Increased Rxfp-1 expression was observed in the sagittal sutures in the mid-suture fibrous tissue following RLN treatment. Rxfp-2 was only expressed in the calvarial bone under tensile stimulation and RLN treatment further increased its expression.
Limitations
RLN-liposomes were not detected at any instance under the current experimental conditions. This is a preliminary study and the sample number limits the power of its results. VVG staining cannot quantify collagen contents but can provide preliminary information on the presence of collagen fibres.
Conclusions
RLN treatment may modify bone remodelling and collagen metabolism during and after suture expansion.
DOI：
10.1016/j.ajodo.2011.07.024
发表时间：
2012-02-01
影响因子：
3
作者：
McGorray, Susan P.;Dolce, Calogero;Wheeler, Timothy T.
通讯作者：
Wheeler, Timothy T.