Proteomic changes in rice leaves grown under open field high temperature stress conditions
Proteomic changes in rice leaves grown under open field high temperature stress conditions
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DOI：
10.1007/s11033-015-3923-5
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发表时间：
2015-11-01
影响因子：
2.8
通讯作者：
Singh, N. K.
中科院分区：
文献类型：
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作者：
Das, Smruti;Krishnan, P.;Singh, N. K.
温度与其他气候和土壤因子的交互作用对水稻的生长发育有着深远的影响。水稻在田间条件下对高温的响应比在控制条件下更重要。为了了解与高温胁迫反应和耐受性相关的基因，所有这些与水稻适应和耐受性相关的基因的表达都需要进行蛋白质组学分析。耐高温应力cvN22在花期分别受到28/18℃（对照）和42/32℃（高温）胁迫。在自由空气增温条件下进行大田栽培。讨论了高温胁迫下水稻叶片蛋白质组学的变化。叶片蛋白质组约有3000个蛋白点，在二维电泳凝胶上可重复检测到，其中573个蛋白在对照和高温处理之间存在差异表达。推测的生理功能包括生长蛋白（15.4%）、热休克蛋白（7.7%）、调节蛋白（26.9%）、氧化还原稳态蛋白（11.5%）和能量代谢相关蛋白（38.5%）等5类。本研究结果表明，cv。N22是农艺学上公认的耐高温水稻品种，其应对高温胁迫的方式十分复杂。几种功能蛋白在其反应中起重要作用。预测的气候变化事件需要在不同的模拟生态条件下对该品种进行更多的研究，以确定蛋白质组学变化和相关基因作为生物标志物，并更好地了解与耐受性相关的生化途径。
The interactive effect of temperature with other climatic and soil factors has profound influences on the growth and development of rice. The responses of rice to high temperatures under field conditions are more important than those under the controlled conditions. To understand the genes associated with high temperature stress response in general and tolerance in particular, the expression of all those genes associated with adaptation and tolerance in rice requires proteomic analysis. High temperature stress-tolerant cv. N22 was subjected to 28/18 A degrees C (control) and 42/32 A degrees C (high temperature stress) at flowering stage. The plants were grown in the field under the free air temperature increment condition. The proteomic changes in rice leaves due to high temperature stress were discussed. The proteomes of leaves had about 3000 protein spots, reproducibly detected on 2-dimensional electrophoretic gels with 573 proteins differentially expressed between the control and the high temperature treatments. Putative physiological functions suggested five categories such as growth (15.4 %), heat shock proteins (7.7 %), regulatory proteins (26.9 %), redox homeostasis proteins (11.5 %) and energy and metabolism (38.5 %) related proteins. The results of the present study suggest that cv. N22, an agronomically recognized temperature tolerant rice cultivar copes with high temperature stress in a complex manner. Several functional proteins play important roles in its responses. The predicted climate change events necessitate more studies using this cultivar under different simulated ecological conditions to identify proteomic changes and the associated genes to be used as biomarkers and to gain a better understanding on the biochemical pathways involved in tolerance.