Higher order structure of chromatin: evidence from photochemically detected linear dichroism.
Higher order structure of chromatin: evidence from photochemically detected linear dichroism.
复制标题
染色质的高阶结构：光化学检测的线性二色性的证据。
DOI：
10.1021/bi00359a052
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Crothers,DM
中科院分区：
文献类型：
--
作者：
Sen,D;Mitra,S;Crothers,DM
耶鲁大学化学系，纽黑文，康涅狄格州06511接收1985年9月3日;修订Mandarin pt接收1986年1月3日摘要：我们已经使用光化学检测线性二色性来测量不同连接体大小（20 - 80个碱基对）的30 nm染色质纤维中核小体和连接体DNA的单独平均角取向。我们的研究结果表明，平均倾斜角随接头大小而变化，但不是以单调的方式，这表明30 nm纤维的几何形状的恒定性是由核小体倾斜的补偿性变化来维持的，核小体倾斜的补偿性变化可容纳接头DNA的可变长度的包装。我们讨论了我们的结果与已提出的30 nm光纤的各种类型的模型的兼容性，包括连续螺线管模型和模型建立的基本单位的锯齿形丝带。许多模型可以消除，所有的修改，以适应我们的结果与很长的连接染色质。
Department of Chemistry, Yale University, New Haven, Connecticut 06511 Received September 3, 1985; Revised Manuscript Received January 3, 1986 abstract: We have used photochemically detected linear dichroism to measure the separate average angular orientations of nucleosomes and linker DNA in 30-nm chromatin fibers of varying linker size (20-80 base pairs). Our results indicate that the average tilt angles vary with linker size, but not in a monotonic manner, suggesting that the constancy of geometry of the 30-nm fiber is maintained by compensatory changes of nucleosomal tilt which accommodate packing of variable lengths of linker DNA. We discuss the compatibility of our results with the various classes of models that have been proposed for the 30-nm fiber, including the continuous solenoid model and models built from the basic unit of the zig-zag ribbon. Many models can be eliminated, and all have to be modified to fit our results for chromatins with verylong linkers.