Prevalence of saliva immunoglobulin A antibodies reactive with severe acute respiratory syndrome coronavirus 2 among Japanese people unexposed to the virus.
Prevalence of saliva immunoglobulin A antibodies reactive with severe acute respiratory syndrome coronavirus 2 among Japanese people unexposed to the virus.
复制标题
在未接触过严重急性呼吸综合征冠状病毒 2 的日本人中，唾液免疫球蛋白 A 抗体的流行率。
DOI：
10.1111/1348-0421.13011
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发表时间：
2022
影响因子：
2.6
通讯作者：
Yamaguchi H
中科院分区：
文献类型：
--
作者：
Tsukinoki K;Yamamoto T;Saito J;Sakaguchi W;Iguchi K;Inoue Y;Ishii S;Sato C;Yokoyama M;Shiraishi Y;Kato N;Shimada H;Makabe A;Saito A;Tanji M;Nagaoka I;Saruta J;Yamaguchi T;Kimoto S;Yamaguchi H
虽然由严重急性呼吸系统综合征冠状病毒2型（SARS-CoV-2）引起的COVID-19大流行对公共卫生构成威胁，因为全球病例和COVID-19相关死亡人数正在增加，但与许多其他国家相比，日本的病毒感染率极低。为了解释这种不寻常的现象，我们调查了日本人中天然存在的（“天然”）抗体的患病率，重点是分泌型免疫球蛋白A（sIgA）形式，与SARS-CoV-2反应。180名年龄范围广泛的健康日本志愿者参与了本研究，他们被认为未暴露于SARS-CoV-2。分别从所有180名参与者和139名成人（年龄≥ 20岁）中采集唾液样本和血液样本。使用酶联免疫吸附试验测定与SARS-CoV-2刺突蛋白-1亚基蛋白的受体结合结构域反应的唾液伊加抗体（主要包括sIgA抗体）以及血清伊加和免疫球蛋白G抗体。主要发现是，52.78%（95%置信区间，45.21%-60.25%）的未接触过SARS-CoV-2的个体对唾液伊加抗体呈阳性，其水平范围在0.002至3.272 ng/mL之间，并且根据年龄，抗体阳性可能呈负趋势。正如我们预期的那样，在研究的日本受试者人群中观察到与SARS-CoV-2反应的可检测的“天然”sIgA抗体频繁发生。
While the COVID‐19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) poses a threat to public health as the number of cases and COVID‐19‐related deaths are increasing worldwide, the incidence of the virus infection is extremely low in Japan compared with many other countries. To explain this uncommon phenomenon, we investigated the prevalence of naturally occurring (“natural”) antibodies, focusing on those of the secretory immunoglobulin A (sIgA) form, reactive with SARS‐CoV‐2 among Japanese people. One hundred and eighty healthy Japanese volunteers of a wide range of age who had been considered to be unexposed to SARS‐CoV‐2 participated in this study. Saliva samples and blood samples were collected from all of the 180 participants and 139 adults (aged ≥ 20 years) included therein, respectively. The determination of saliva IgA antibodies, mostly comprising sIgA antibodies, as well as serum IgA and immunoglobulin G antibodies, reactive with the receptor binding domain of the SARS‐CoV‐2 spike‐1 subunit proteins was conducted using an enzyme‐linked immunosorbent assay. The major findings were that 52.78% (95% confidence interval, 45.21%–60.25%) of the individuals who had not been exposed to SARS‐CoV‐2 were positive for saliva IgA antibodies with a wide range of levels between 0.002 and 3.272 ng/mL, and that there may be a negative trend in positivity for the antibodies according to age. As we had expected, a frequent occurrence of assumable “natural” sIgA antibodies reactive with SARS‐CoV‐2 among the studied Japanese participant population was observed.