DNA methylation by N-methyl-N-nitrosourea: methylation pattern changes in single- and double-stranded DNA, and in DNA with mismatched or bulged guanines.
DNA methylation by N-methyl-N-nitrosourea: methylation pattern changes in single- and double-stranded DNA, and in DNA with mismatched or bulged guanines.
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N-甲基-N-亚硝基脲造成的 DNA 甲基化：单链和双链 DNA 以及鸟嘌呤不匹配或凸出的 DNA 中的甲基化模式发生变化。
DOI：
10.1093/nar/21.21.4975
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发表时间：
1993
影响因子：
14.9
通讯作者：
Gold,B
中科院分区：
文献类型：
--
作者：
Wurdeman,RL;Douskey,MC;Gold,B
使用 N-甲基-N-亚硝基脲 (MNU) 和 2,12,-二甲基-3,7,11,17-四氮杂双环-[11.3.1]heptadeca- 1-[17],2,11,13,15 戊烯-Ni (II) 在合成 32P 末端标记的脱氧核糖核苷酸寡聚体中对异常 DNA 碱基配对排列和构象进行化学探测（镍络合物）与 KHSO5。研究的 DNA 靶标是单链 (s-s) DNA、双链 (d-s) DNA、具有 G-G、G-A 和 G-T 错配的 d-s DNA、具有单个凸出 G 的 d-s DNA 和具有两个凸出 G 的 d-s DNA。报告了非沃森-克里克结构对 MNU 形成 N7-甲基鸟嘌呤 (N7-MeG) 和 Ni 络合物氧化 G 的影响，以及不同双链低聚物的 Tm 和圆二色光谱。 MNU 和 Ni 复合物的结果表明，G3run 裂解模式的定性和定量特征随异常碱基配对基序的性质而变化。基于所研究的 DNA 底物，结果表明报告电子和空间扰动的试剂组合可以成为监测 DNA 错配和凸起的有用方法。
The detection of abnormal DNA base pairing arrangements and conformations is chemically probed in synthetic32P-end-labeled deoxyribonucleotide oligomers using N-methyl-N-nitrosourea (MNU) and 2,12,-dimethyl-3,7,11,17-tetraazabicyclo-[11.3.1]heptadeca- 1-[17],2,11,13,15 pentaene-Ni (II) (Ni-complex) with KHSO5. The DNA targets studied are singlestranded (s-s) DNA, double-stranded (d-s) DNA, d-s DNA with G-G, G-A and G-T mismatches, d-s DNA with a single bulged G and d-s DNA with two bulged G's. The effect of the non-Watson–Crick structures on the formation of N7-methylguanine (N7-MeG) by MNU and the oxidation of G by Ni-complex is reported along with the Tm′s and circular dichroism spectra of the different duplex oligomers. The results for MNU and Ni-complex show that the qualitative and quantitative character of the cleavage patterns at a G3run change with the nature of the abnormal base pairing motif. Based on the DNA substrates studied, the results indicate that a combination of reagents which report electronic and steric perturbations can be a useful approach to monitor DNA mismatches and bulges.