Autophagy plays a potential role in the process of sea cucumber body wall “melting” induced by UV irradiation
Autophagy plays a potential role in the process of sea cucumber body wall “melting” induced by UV irradiation
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DOI：
10.1007/s11859-008-0220-3
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发表时间：
2008-07
影响因子：
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通讯作者：
B. Zhu;Jie Zheng;Zong-shen Zhang;Xiu‐ping Dong;Lulu Zhao;M. Tada
中科院分区：
文献类型：
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作者：
B. Zhu;Jie Zheng;Zong-shen Zhang;Xiu‐ping Dong;Lulu Zhao;M. Tada
研究了海参体壁在紫外线诱导下“融化”过程中组织形态和结构的变化。并对酸性磷酸酶（ACP）、组织蛋白酶B和组织蛋白酶L的活性进行了定位和测定。结果表明，胶原纤维束断裂，结缔组织出现空洞，胶原纤维束断裂成不规则碎片。核膜染色质边缘化。高尔基体和内质网均肿胀、卷曲，最终形成双层或多层囊泡。自噬小泡大量分布于整个细胞质中。ACP在UV照射后变得更活跃。组织蛋白酶B和组织蛋白酶L的活性在一定条件下均达到最大值。这表明自噬在紫外线诱导的海参体壁“融化”过程中起着潜在的作用。
The changes of tissue appearances and structures in the process of UV-induced “melting” for sea cucumber (Stichopus japonicus) body wall were studied. And the localization and determination of acid phosphatase (ACP), Cathepsin B and Cathepsin L activities were also investigated. The results show that the connective tissue was damaged with many hollows emerging and the regular collagen bundles were broken apart into irregular fragments. Margination of condensed chromatin at the nuclear membrane was observed. Both Golgi’s body and endoplasmic reticulum swelled, curled, and eventually double-or multi-lamellar vesicles were formed. A number of autophagic vesicles distributed in all through the whole cytoplasm. ACP becomes more active after UV irradiation. The activities of cathepsin B and cathepsin L increased in UV-treated sea cucumbers and both achieved their maximum under certain conditions. It indicates that autophagy plays a potential role in the “melting” process for sea cucumber body wall induced by UV irradiation.