Elemental and redox analysis of single bacterial cells by X-ray microbeam analysis
Elemental and redox analysis of single bacterial cells by X-ray microbeam analysis
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DOI：
10.1126/science.1103524
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发表时间：
2004-10-22
期刊：
影响因子：
56.9
通讯作者：
Nealson, KH
中科院分区：
文献类型：
--
作者：
Kemner, KM;Kelly, SD;Nealson, KH
高能 X 射线荧光测量用于制作单个荧光假单胞菌菌株 NCIMB 11764 细胞的元素图和定性化学分析。浮游细胞和贴壁细胞在形态、元素组成和水合细胞在 150 nm 空间尺度上对 Cr(VI) 的敏感性方面存在显着差异。该技术可应用于自然地球微生物系统。
High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity to Cr(VI) of hydrated cells at spatial scales of 150 nm. This technology can be applied to natural geomicrobiological systems.