HUMAN AMNION MEMBRANE SERVES AS A SUBSTRATUM FOR GROWING AXONS INVITRO AND INVIVO
HUMAN AMNION MEMBRANE SERVES AS A SUBSTRATUM FOR GROWING AXONS INVITRO AND INVIVO
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DOI：
10.1126/science.3576223
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发表时间：
1987-05-29
期刊：
影响因子：
56.9
通讯作者：
GAGE, FH
中科院分区：
文献类型：
--
作者：
DAVIS, GE;BLAKER, SN;GAGE, FH
人羊膜上皮细胞层可以在基底膜和间质表面形态完整的情况下被切除。这种制剂已被用作离体培养游离神经元的基质。鸡睫状神经节的胚胎运动神经元附着于两个表面，但仅在基底膜上生长出广泛的神经突。在羊膜的横截面上，培养神经元的再生轴突被引导沿着基膜的途径，基膜对层粘连蛋白抗体有免疫反应。此外，将卷起的羊膜植入大鼠中隔和海马之间的病变腔内，胆碱能神经元通过纵向定向植入到海马中延伸轴突。因此，这种羊膜制剂可以作为促进受损成人大脑轴突再生的桥梁。
The epithelial cell layer of human amnion membrane can be removed while the basement membrane and stromal surfaces remain morphologically intact. Such a preparation has been used as a substratum for the in vitro culture of dissociated neurons. Embryonic motor neurons from chick ciliary ganglion attached to both surfaces but grew extensive neurites only on the basement membrane. On cross sections of rolled amnion membranes, regenerating axons of cultured neurons were guided along pathways of basement membrane that were immunoreactive with an antibody to laminin. In addition, when rolled amnion membranes were implanted into a lesion cavity between the rat septum and hippocampus, cholinergic neurons extended axons through the longitudinally oriented implant into the hippocampus. Thus, this amnion preparation can serve as a bridge to promote axonal regeneration in vivo in damaged adult brain.