The Inhibitory Effect of a Macrocyclic Bisbibenzyl Riccardin D on the Biofilms of Candida albicans
The Inhibitory Effect of a Macrocyclic Bisbibenzyl Riccardin D on the Biofilms of Candida albicans
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大环双联苄基 Riccardin D 对白色念珠菌生物膜的抑制作用
DOI：
10.1248/bpb.32.1417
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发表时间：
2009-08-01
影响因子：
2
通讯作者：
Lou, Hongxiang
中科院分区：
文献类型：
--
作者：
Cheng, Aixia;Sun, Lingmei;Lou, Hongxiang
生物膜的形成在许多微生物的生命周期和生存中起着关键作用。人类真菌病原体白色念珠菌非常容易形成生物膜，并对传统抗真菌药物产生耐药性。生物膜由多种细胞类型的混合物组成，包括酵母、假菌丝和菌丝细胞，菌丝是生物膜的一个显着特征。 Riccardin D 是我们实验室从地草 Dumortiera hirsute 中分离出来的大环双联苯甲基。在本研究中，采用 XTT [2,3-双（2-甲氧基-4-硝基-5-磺基-苯基）-2H-四唑鎓-5-甲酰苯胺]还原测定和活/死细胞染色来评估 riccardin D 对白色念珠菌生物膜的影响。结果表明，riccardin D 可以干扰生物膜的形成。为了探讨这种作用是否是由于抑制菌丝形成所致，采用形态学观察和实时逆转录聚合酶链反应（RT-PCR）来评估riccardin D对菌丝形成和菌丝特异性基因表达的影响。结果表明，riccardin D处理后菌丝形成受到强烈抑制，菌丝特异性基因的mRNA表达水平下调。我们得出结论，riccardin D 通过下调菌丝特异性基因的表达并抑制菌丝的形成来干扰白色念珠菌生物膜的形成。
Biofilm formation plays a key role in the life cycles and subsistence of many microorganisms. The human fungal pathogen Candida albicans has a high propensity to develop biofilms and resulted resistant to traditional antifungal agents. Biofilms are composed of a mixture of cell types, including yeast, pseudohyphal and hyphal cells, and hyphae are a prominent feature of biofilms. Riccardin D is a macrocyclic bisbibenzyl isolated from the liverwort Dumortiera hirsute in our laboratory. In the present investigation, the XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] reduction assay and live/dead cell staining were employed for evaluating the effects of riccardin D on C albicans biofilms. The results demonstrated that riccardin D can interfere with the biofilm formation. To investigate whether this effect was due to the inhibition of hyphae formation, morphological observation and real-time reverse transcriptase polymerase chain reaction (RT-PCR) were employed for evaluating the effects of riccardin D on the hyphae formation and the expression of hyphae specific genes. The results showed that the hyphae formation was strongly inhibited and the mRNA expression levels of hyphae specific genes were downregulated after riccardin D treatment. We concluded that riccardin D interfered with the biofilm formation of C. albicans through downregulating the expression of hyphae specific genes and inhibiting the formation of hyphae.