Three distinct signals can induce class II gene expression in a murine pre-B-cell line.
Three distinct signals can induce class II gene expression in a murine pre-B-cell line.
复制标题
三种不同的信号可以在小鼠前 B 细胞系中诱导 II 类基因表达。
DOI：
10.1073/pnas.83.13.4878
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Glimcher,LH
中科院分区：
文献类型：
--
作者：
Polla,BS;Poljak,A;Geier,SG;Nathenson,SG;Ohara,J;Paul,WE;Glimcher,LH
主要组织相容性复合体（MHC）的II类基因的表达已经在Abelson-鼠白血病病毒转化的前B细胞系R8及其II类分子（Ia）阴性变体R8205中进行了研究。这些变异细胞含有几乎检测不到的水平的RNA特异性的所有II类基因，包括非多态性不变链基因（Ii），并不表达细胞表面Ia。这种鼠Ia阴性细胞系与人Ia阳性Raji细胞的融合产生了表达鼠Ia的种间杂交瘤。这些数据进一步证明了存在可以调节II类基因表达的反式作用因子。此外，T细胞衍生的淋巴因子B细胞刺激因子1（BSF-1）诱导R8205细胞中II类基因的表达。将R8205细胞暴露于已显示模拟BSF-1对正常B细胞的活性的抗体也导致II类基因的表达。这些数据表明，三种不同的信号-淋巴因子，同种抗体结合膜结构，和种间反式作用因子-可以诱导II类基因的表达。
Expression of class II genes of the major histocompatibility complex (MHC) has been studied in an Abelson-murine-leukemia-virus-transformed pre-B-cell line, R8, and its class II molecule (Ia)-negative variant, R8205. These variant cells contained barely detectable levels of RNA specific for all class II genes, including the nonpolymorphic invariant chain gene (Ii), and did not express cell surface Ia. Fusion of this murine Ia-negative cell line to the human Ia-positive Raji cell produced an interspecies hybridoma that expressed the murine Ia. These data are further evidence for the existence of a trans-acting factor(s) that can regulate class II gene expression. Furthermore, the T-cell-derived lymphokine B-cell-stimulatory factor 1 (BSF-1) induced expression of class II genes in the R8205 cells. Exposure of R8205 cells to an antibody that has been shown to mimic BSF-1 activity on normal B cells also resulted in expression of class II genes. These data demonstrate that three distinct signals--a lymphokine, an alloantibody binding to membrane structures, and an interspecies trans-acting factor--can induce expression of class II genes.