Identification of nanometre-sized ultra-high molecular weight polyethylene wear particles in samples retrieved in vivo
Identification of nanometre-sized ultra-high molecular weight polyethylene wear particles in samples retrieved in vivo
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DOI：
10.1302/0301-620x.90b8.20737
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发表时间：
2008-08-01
影响因子：
--
通讯作者：
Tipper, J. L.
中科院分区：
文献类型：
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作者：
Richards, L.;Brown, C.;Tipper, J. L.
从髋关节模拟器中回收的润滑剂中发现了超高分子量聚乙烯的纳米级颗粒。从7例失败的Chamley全髋关节置换术中采集组织样本，使用强碱消化，并使用高分辨率场发射枪扫描电子显微镜进行分析，以确定体内是否产生纳米级聚乙烯碎片颗粒。一种随机的分析方法被用来量化和分析所有分离的聚乙烯颗粒。我们从回收的组织样本中分离出纳米尺寸的颗粒。确定的最小为30 nm，大多数在0.1 μ m至0.99 μ m的尺寸范围内。1.0 μ m至9.99 μ m尺寸范围内的颗粒代表了组织样本磨损体积的最高比例，总磨损体积的35%至98%由该尺寸的颗粒组成。从组织中分离出的纳米级颗粒数量仅占总磨损体积的一小部分。需要进一步的工作来评估纳米聚乙烯颗粒的生物反应。
Nanometre-sized particles of ultra-high molecular weight polyethylene have been identified in the lubricants retrieved from hip simulators. Tissue samples were taken from seven failed Chamley total hip replacements, digested using strong alkali and analysed using high-resolution field emission gun-scanning electron microscopy to determine whether nanometre-sized particles of polyethylene debris were generated in vivo. A randomised method of analysis was used to quantify and characterise all the polyethylene particles isolated.We isolated nanometre-sized particles from the retrieved tissue samples. The smallest identified was 30 nm and the majority were in the 0.1 mu m to 0.99 mu m size range. Particles in the 1.0 mu m to 9.99 mu m size range represented the highest proportion of the wear volume of the tissue samples, with 35% to 98% of the total wear volume comprised of particles of this size. The number of nanometre-sized particles isolated from the tissues accounted for only a small proportion of the total wear volume. Further work is required to assess the biological response to nanometre-sized polyethylene particles.