The myth of ventrally emigrating neural tube (VENT) cells and their contribution to the developing cardiovascular system
The myth of ventrally emigrating neural tube (VENT) cells and their contribution to the developing cardiovascular system
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DOI：
10.1007/s00429-002-0302-5
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发表时间：
2003-03-01
期刊：
影响因子：
--
通讯作者：
Poelmann, RE
中科院分区：
文献类型：
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作者：
Boot, MJ;Gittenberger-de Groot, AC;Poelmann, RE
心脏神经嵴细胞是一组细胞，在特定的时间窗口内从神经管的背侧迁移，并有助于咽弓动脉和心脏的主动脉肺隔。最近的出版物表明，另一组从神经管腹侧迁移的细胞也有助于心血管系统的发育。本研究的第一个目的是通过将含有lacZ报告基因的逆转录病毒注射到发育不同阶段的鸡胚中，确定心脏神经嵴细胞迁移的特定时间窗。第二个目的是使用三种方法研究假定的腹侧迁移神经管细胞对心血管系统的贡献。一种方法是将一种lacZ逆转录病毒注射到鸡后脑的管腔中。其次，我们将逆转录病毒注射到神经管10-12染色体对的位置。最后，我们使用了嵌合体技术，将鹌鹑神经管段移植到小鸡胚胎中。心脏神经嵴细胞在HH9和HH13（-）之间从神经管背侧迁移。HH13（+）神经管已停止产生心脏神经嵴细胞的水平之间的位置和第四对体。将逆转录病毒直接注射到HH14时的鸡后脑中，50%的胚胎后脑标记极少，相邻间质标记强烈，表明病毒溢出。这意味着该技术对于确认腹侧迁移细胞的存在是无用的。将逆转录病毒注射到10-12体对位置的HH14神经管腔和嵌合体均未显示神经管细胞向腹侧迁移的迹象。我们得出结论，腹侧神经管细胞对心血管系统的发育没有贡献。
The cardiac neural crest cells are a group of cells that emigrate from the dorsal side of the neural tube during a specific time window and contribute to the pharyngeal arch arteries and the aorticopulmonary septum of the heart. Recent publications have suggested that another group of cells emigrating from the ventral side of the neural tube also contributes to the developing cardiovascular system. The first aim of our study was to define the specific time window of cardiac neural crest cell migration by injecting a retrovirus containing a lacZ reporter gene into a chick embryo at different stages during development. The second aim was to study the contribution of the supposed ventrally emigrating neural tube cells to the cardiovascular system using three approaches. One approach was to inject a lacZ retrovirus into the lumen of the chick hindbrain. Secondly, we injected the retrovirus into the neural tube at the position of the 10-12 somite pair. Finally, we used the chimera technique in which we transplanted a quail neural tube segment into a chick embryo. Cardiac neural crest cells were shown to emigrate from the dorsal side of the neural tube between HH9 and HH13(-). The HH13(+) neural tube has ceased to produce cardiac neural crest cells between the level of the otic placode and the fourth pair of somites. Retroviral injection directly into the chick hindbrain at HH14 resulted in 50% of the embryos with minimal labeling of the hindbrain and intense labeling of the adjacent mesenchyme, suggesting that virus was spilled. This implies that this technique is not useful for confirming the existence of ventrally emigrating cells. Both retroviral injections into the neural tube lumen at HH14 at the position of the 10-12 somite pair and the chimeras showed no signs of ventrally emigrating neural tube cells. We conclude that there is no contribution of ventral neural tube cells to the developing cardiovascular system.