Carboxylate Ionic Liquids Combining Low Cytotoxicity toward HepG2 Cell and High Separation Efficiency for Bioactive Molecules
Carboxylate Ionic Liquids Combining Low Cytotoxicity toward HepG2 Cell and High Separation Efficiency for Bioactive Molecules
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羧酸盐离子液体结合了对 HepG2 细胞的低细胞毒性和对生物活性分子的高分离效率。
DOI：
10.1021/acssuschemeng.6b02778
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发表时间：
2017-02-01
影响因子：
8.4
通讯作者：
Xing, Huabin
中科院分区：
文献类型：
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作者：
Liu, Xianxian;Ji, Cai;Xing, Huabin
离子液体由于其独特的物理化学性质，在药物相关过程中作为溶剂受到了广泛的关注。然而，目前关于IL对哺乳动物细胞系的细胞毒性的研究还很不足，开发高效、低毒的IL仍然是一个巨大的挑战。本文首次报道了羧酸胆碱离子液体对HepG 2细胞的细胞毒性及其对天然生物活性分子的分离性能。首先，通过MTT细胞活力测定系统评价了这些IL对HepG 2细胞的细胞毒性，然后通过生育酚同系物的萃取分离实验考察了它们作为萃取剂的功能。结果表明，与广泛使用的咪唑鎓氯化物离子液体相比，这些离子液体结合了较低的细胞毒性和较高的性能。例如，乙酸胆碱的IC 50为135.53，约为1-乙基胆碱的4倍。
Ionic liquids (ILs) have attracted a great deal of attention as solvents in drug-related processes because of their unique physicochemical properties. However, investigations on the cytotoxicity of ILs toward mammalian cell lines are quite insufficient, and it remains a great challenge to develop ILs with both higher efficiency and less cytotoxicity. Herein we reported the cytotoxicity of cholinium carboxylate ILs toward HepG2 cells and their separation performance for natural bioactive molecules for the first time. At first, the cytotoxicity toward HepG2 cells of these ILs was systematically evaluated by MTT cell viability assays, and then their functionality as extractants was investigated through the extractive separation experiments of tocopherol homologues. The results demonstrated that these ILs combined both lower cytotoxicity and higher performance than the widely used imidazolium chloride ILs. For example, the IC50 of cholinium acetate was 135.53, approximately 4 times as large as that of 1-ethyl...