Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact
Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact
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DOI：
10.1111/hel.12138
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发表时间：
2014-10-01
期刊：
影响因子：
4.4
通讯作者：
Cao, Yi
中科院分区：
文献类型：
--
作者：
Zhang, Chen;Zhang, Hongyu;Cao, Yi
上皮连接和粘蛋白是构成粘膜屏障的重要组成部分。幽门螺杆菌（H.pylori）感染诱导上皮细胞的紧密连接和粘附连接的改变，虽然这个过程的确切机制尚未完全understood.MethodsThe粘附分子和MUC1的表达进行了系统的研究，在胃肠道上皮细胞感染幽门螺杆菌在体外和体内。此外，我们开发了几种新的体外方法来研究细菌和紧密连接功能障碍之间的关系，使用Boyden Chambers.ResultsThe表达的一系列连接分子和MUC1在培养的细胞，感染幽门螺杆菌。根据紧密连接处的损伤程度，与幽门螺杆菌与基底膜结合或不结合细胞相比，幽门螺杆菌与细胞顶膜的直接接触导致渗透性的最大增加。结论幽门螺杆菌可直接导致胃肠道黏膜屏障的严重损伤，基于Boyden小室系统的新方法可用于研究细菌与其靶细胞之间的关系。
BackgroundEpithelial junctions and mucins compose a major portion of the mucosal barrier. Helicobacter pylori (H.pylori) infections induce alterations of the tight junctions and adherens junctions in epithelial cells, although the precise mechanisms underlying this process are not fully understood.MethodsThe expression of adhesion molecules and MUC1 was systematically investigated in gastrointestinal epithelial cells infected with H.pylori in vitro and in vivo. Furthermore, we developed several new in vitro methods to study the relationships between the bacterium and the dysfunction of tight junctions using Boyden Chambers.ResultsThe expression of a series of junctional molecules and MUC1 decreased in the cultured cells that were infected with H.pylori. According to the degree of damage at the tight junctions, direct contact of H.pylori with the apical membrane of the cells resulted in the greatest increase in permeability compared to basal membrane binding or non-binding of H.pylori to the cells. Similarly, we noted that H.pylori infection could reduce the expression and glycosylation of MUC1.ConclusionsHelicobacter pylori dwelling on the apical surface of the gastrointestinal epithelium could directly induce serious injury of the mucosal barrier, and the new methods outlined here, based on the Boyden Chamber system, could be very useful for studying the relationships between bacteria and their target cells.