PROTECTIVE PROPERTIES OF EGG-YOLK IGY CONTAINING ANTI-EDWARDSIELLA-TARDA ANTIBODY AGAINST PARACOLO DISEASE IN THE JAPANESE EEL, ANGUILLA-JAPONICA TEMMINCK AND SCHLEGEL
PROTECTIVE PROPERTIES OF EGG-YOLK IGY CONTAINING ANTI-EDWARDSIELLA-TARDA ANTIBODY AGAINST PARACOLO DISEASE IN THE JAPANESE EEL, ANGUILLA-JAPONICA TEMMINCK AND SCHLEGEL
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DOI：
10.1111/j.1365-2761.1993.tb00854.x
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发表时间：
1993-03-01
影响因子：
2.5
通讯作者：
KIM, M
中科院分区：
文献类型：
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作者：
GUTIERREZ, MA;MIYAZAKI, T;KIM, M
已知免疫母鸡在其蛋黄中含有高水平的免疫球蛋白Y（IgY）。本研究用福尔马林灭活的鸡卵黄蛋白免疫母鸡，获得了抗迟缓爱德华氏菌IgY（含20%特异性IgY，凝集效价1：128）。该IgY对鳗鱼消化因子具有稳定性，因此可与活的E.迟缓症对日本鳗鱼的影响及对迟缓症的预防效果。评估迟发性感染。以400 mg fish-1清除的E.在24小时内从肠内同时接种10（5-6）CFU fish-1。口服200和400 mgfish-1的IgY可抑制E.将10（4-6）CFU fish-1的tarda通过受损的肠同时接种到肝脏和肾脏中。口服给予IgY的鱼显示死亡率降低。这些结果表明，含有抗E。迟发型IgY对爱德华氏菌病有较好的预防作用。
Immunized hens are known to contain a high level of immunoglobulin Y (IgY) in their egg yolk. In this study, the present authors obtained anti-Edwardsiella tarda IgY (containing 20% specific IgY, agglutination titre, 1:128) from hens vaccinated by injection with formalin-killed bacterin. The IgY was stable against eel digestive factors, and therefore, was orally administered with viable E. tarda to the Japanese eels and the efficacy of protection against E. tarda infection was evaluated. Orally administered IgY at a dose of 400 mg fish-1 cleared E. tarda inoculated simultaneously at 10(5-6) CFU fish-1 from the intestine within 24h. Moreover, orally administered IgY at doses of 200 and 400mg fish-1 inhibited the penetration of E. tarda inoculated simultaneously at 10(4-6) CFU fish-1 into the liver and kidney via the damaged intestine. The fish orally administered with IgY showed reduced mortality. These results suggest that egg yolk containing anti-E. tarda IgY is effective in preventing edwardsiellosis.