Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI
Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI
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DOI：
10.1016/s0092-8674(00)80379-7
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发表时间：
1997-09-19
期刊：
影响因子：
64.5
通讯作者：
Kreis, TE
中科院分区：
文献类型：
--
作者：
Scales, SJ;Pepperkok, R;Kreis, TE
使用水泡性口炎病毒的温度敏感性糖蛋白和绿色荧光蛋白（ts-G-GFP（ct））的嵌合体，在活细胞中已经观察到从内质网（ER）到高尔基复合体的胞吐运输。在转变到允许温度时，ts-G-GFP（ct）浓缩到靠近ER的COPII阳性结构中，然后形成含有ERGIC-53和KDEL受体的中间室或转运复合物，其中COPII被COPI取代。这些结构出现异质性和移动微管依赖的方式向高尔基复合体。我们的研究结果表明，COPII和COPI行动的顺序模式，并表明，运输复合物的ER-高尔基体运输中间体，COPI可能参与回收材料的ER。
Exocytic transport from the endoplasmic reticulum (ER) to the Golgi complex has been visualized in living cells using a chimera of the temperature-sensitive glycoprotein of vesicular stomatitis virus and green fluorescent protein (ts-G-GFP(ct)). Upon shifting to permissive temperature, ts-G-GFP(ct) concentrates into COPII-positive structures close to the ER, which then build up to form an intermediate compartment or transport complex, containing ERGIC-53 and the KDEL receptor, where COPII is replaced by COPI. These structures appear heterogenous and move in a microtubule-dependent manner toward the Golgi complex. Our results suggest a sequential mode of COPII and COPI action and indicate that the transport complexes are ER-to-Golgi transport intermediates from which COPI may be involved in recycling material to the ER.