TRYPANOSOMA-BRUCEI - RAPID MATCHING METHOD FOR ESTIMATING HOSTS PARASITEMIA
TRYPANOSOMA-BRUCEI - RAPID MATCHING METHOD FOR ESTIMATING HOSTS PARASITEMIA
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DOI：
10.1016/0014-4894(76)90110-7
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发表时间：
1976-01-01
影响因子：
2.1
通讯作者：
LUMSDEN, WHR
中科院分区：
文献类型：
--
作者：
HERBERT, WJ;LUMSDEN, WHR
一种估计锥虫感染中寄生虫血症绝对水平的新技术[例如，在小鼠中]描述。在较高的感染水平下，这是通过将湿血膜的显微镜视野与图表相匹配来实现的，并且在存在较少生物体的情况下，通过在5、10或20个这样的显微镜视野中计数锥虫的数量来实现。可以在显微镜下观察到的寄生虫血症的整个范围内迅速对每毫升血液中的微生物数量进行良好的估计，即，高于反对数5.4（250，000）个微生物/ml。
A new technique for estimating the absolute level of parasitemias in trypanosome infections [e.g., in mice] is described. At higher levels of infection this is achieved by matching microscopic fields of a wet blood film against charts and, where fewer organisms are present, by counting the number of trypanosomes in 5,10 or 20 such microscope fields. Good estimates of the number of organisms per milliliter of blood can be made rapidly over the whole range of microscopically patent parasitemia, i.e., above antilog 5.4 (250,000) organisms/ml.