Room temperature phosphorescent determination of aflatoxins in fish feed based on molecularly imprinted polymer - Mn-doped ZnS quantum dots
Room temperature phosphorescent determination of aflatoxins in fish feed based on molecularly imprinted polymer - Mn-doped ZnS quantum dots
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DOI：
10.1016/j.aca.2019.12.060
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发表时间：
2020-03-22
影响因子：
6.2
通讯作者：
Moreda-Pineiro, Antonio
中科院分区：
文献类型：
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作者：
Madurangika Jayasinghe, G. D. Thilini;Dominguez-Gonzalez, Raquel;Moreda-Pineiro, Antonio
探索了基于分子印迹聚合物纳米传感器的掺锰硫化锌量子点室温光谱分析方法，用于黄曲霉毒素的灵敏和选择性测定。合成的聚合物纳米粒子表现出强烈的室温磷光(总衰减时间为0.004 S)，黄曲霉毒素与连接在磷光量子点上的分子印迹聚合物的识别腔相互作用时，黄曲霉毒素猝灭了室温磷光。在290 nm激发后，扫描520~720 nm(最大峰值强度为594 nm)，记录室温磷光。制备的印迹材料比非印迹量子点具有更高的吸附容量，表现出对黄曲霉毒素的高吸附能力。此外，选择性研究表明，该材料对黄曲霉毒素具有特定的识别作用。黄曲霉毒素猝灭黄曲霉毒素的线性范围为2-20 mg·L-1，检出限为3.56 mg·kg~(-1)。这一数值低于欧盟委员会公布的最大可接受/残留量(饲料中的黄曲霉毒素)。结果表明，一种简单的室温磷光纳米传感器检测鱼饲料中的黄曲霉毒素具有良好的灵敏度和选择性，是一种通用的检测工具。(C)2019爱思唯尔B.V.保留所有权利。
Possibilities of room temperature spectrometry based on Mn-doped ZnS quantum dots coated with a molecularly imprinted polymer based nanosensor have been explored for the sensitive and selective determination of aflatoxins. Synthesized polymeric nanoparticles exhibit intense room temperature phosphorescence (total decay time of 0.004 s) and aflatoxins quench the room temperature phosphorescence when interact with the recognition cavities of the molecularly imprinted polymer attached to the phosphorescent quantum dots. Room temperature phosphorescence was recorded by scanning from 520 nm to 720 nm (maximum peak intensity at 594 nm) after excitation at 290 nm. The prepared imprinted material was found to have higher adsorption capacity than those based non-imprinted quantum dots, demonstrating high adsorption uptake for aflatoxins. In addition, selectivity studies have demonstrated that the material offers a specific recognition for aflatoxins. Room temperature phosphorescence quenching by aflatoxins was found to be linear within the 2-20 mg L-1 range, and a limit of detection of 3.56 mg kg(-1) was obtained. This value was lower than the maximum acceptable/residual level (aflatoxins in feeds) published by the European Commission. The results indicate a simple room temperature phosphorescence nanosensor for aflatoxins detection in fish feed as a versatile tool having excellent sensitivity and selectivity. (c) 2019 Elsevier B.V. All rights reserved.