Measurement of chemical exchange rate constants with solvent protons using radiation damping
Measurement of chemical exchange rate constants with solvent protons using radiation damping
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DOI：
10.1006/jmre.1998.1356
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发表时间：
1998-04-01
影响因子：
2.2
通讯作者：
Mao, XA
中科院分区：
文献类型：
--
作者：
Chen, JH;Mao, XA
当化学交换发生在浓缩物质和稀释物质之间时，如生化系统中水和蛋白质之间的质子交换，很难用常规方法进行定量动力学研究。本文提出了一种利用水强磁化的辐射阻尼与生物分子中移动质子的弱共振交换的新方法。用简单的逆恢复脉冲序列，在稀胍溶液中，通过求解经辐射阻尼和化学交换效应修正的Bloch方程，证明了水和胍质子之间的交换速率常数。(C) 1998学术出版社。
When chemical exchange occurs between a concentrated and a diluted species like the proton exchange between water and proteins in biochemical systems, it is difficult to use the conventional methods for a quantitative dynamical study. In this paper, a new approach which utilizes radiation damping of the strong water magnetization exchanging with the weak resonance of the mobile protons in biomolecules is proposed. It is demonstrated with a dilute guanidine solution, using the simple inversion-recovery pulse sequence, that the exchange rate constants between water and guanidine protons are evaluated by solving the Bloch equations modified with both the radiation damping and the chemical exchange effects. (C) 1998 Academic Press.