Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids.
Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids.
复制标题
过氧化物酶体功能的差异重塑支撑着双克隆酰胺和动质体的环境和代谢适应性。
DOI：
10.1098/rspb.2016.0520
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Nara T.
中科院分区：
文献类型：
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作者：
Morales J;Hashimoto M;Williams TA;Hirawake-Mogi H;Makiuchi T;Tsubouchi A;Kaga N;Taka H;Fujimura T;Koike M;Mita T;Bringaud F;Concepcion JL;Hashimoto T;Embley TM;Nara T.
细胞器功能的重塑越来越被认为是真核生物多样性和进化的核心驱动力。包括锥虫和利什曼虫在内的动质体已经进化出了专门的过氧化物酶体，称为糖体。糖体独特地含有糖酵解途径以及其他酶，这支撑了这些主要人类病原体的生理灵活性。动质体的姐妹群是双酰胺类，它们是海洋浮游生物中最丰富的真核生物之一。在这里，我们证明了在自由生活的海洋diplonemid，Diplonema papillatum的过氧化物酶体中，糖酵解或糖酵解的区室化。我们的研究结果表明，过氧化物酶体修饰已经在动质体和diplonemids的共同祖先进行，并提出了可能性，即在异养自由生活的祖先中，碳代谢的核心重要性可能是一个重要的选择性驱动因素。我们的数据表明，过氧化物酶体修饰并不局限于动质体谱系，但也一直是他们的自由生活的裸藻动物亲属的成功的一个因素。
The remodelling of organelle function is increasingly appreciated as a central driver of eukaryotic biodiversity and evolution. Kinetoplastids includingTrypanosomaandLeishmaniahave evolved specialized peroxisomes, called glycosomes. Glycosomes uniquely contain a glycolytic pathway as well as other enzymes, which underpin the physiological flexibility of these major human pathogens. The sister group of kinetoplastids are the diplonemids, which are among the most abundant eukaryotes in marine plankton. Here we demonstrate the compartmentalization of gluconeogenesis, or glycolysis in reverse, in the peroxisomes of the free-living marine diplonemid,Diplonema papillatum. Our results suggest that peroxisome modification was already under way in the common ancestor of kinetoplastids and diplonemids, and raise the possibility that the central importance of gluconeogenesis to carbon metabolism in the heterotrophic free-living ancestor may have been an important selective driver. Our data indicate that peroxisome modification is not confined to the kinetoplastid lineage, but has also been a factor in the success of their free-living euglenozoan relatives.