Tannic acid alleviates bulk and nanoparticle Nd2O3 toxicityin pumpkin: A physiological and molecular response.
Tannic acid alleviates bulk and nanoparticle Nd2O3 toxicityin pumpkin: A physiological and molecular response.
复制标题
单宁酸减轻南瓜中的块状和纳米颗粒 Nd2O3 毒性：生理和分子反应。
DOI：
10.1080/17435390.2016.1202349
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发表时间：
2016
期刊：
影响因子：
5
通讯作者：
Xing BS
中科院分区：
文献类型：
--
作者：
Guangcai Chen;Chuanxin Ma;Mukherjee A;Musante C;Zhang JF;White JC;Dhankher OP;Xing BS
溶解性有机物（DOM）对纳米颗粒对植物的毒性影响知之甚少。以单宁酸（TA）为DOM替代物，探讨氧化钕纳米颗粒（Nd 2 O3 NPs）对南瓜（Cucurbita maxima）的毒性机制。测试浓度的结果表明，100 mg L− 1 Nd 2 O3 NPs对南瓜的新鲜生物量具有明显的毒性，并且散装颗粒和离子处理的类似结果也很明显。在1/5 Hoagland溶液中暴露于100 mg L-1的Nd 2 O3 NPs和BPs不仅显著抑制南瓜生长，而且降低了植物组织中S，Ca，K和Mg的水平。然而，60 mg L− 1 TA显着缓和所观察到的植物毒性，减少根中Nd的积累，并显着恢复NPs和BPs处理的南瓜中S，Ca，K和Mg的水平。60 mg L-1的TA使暴露于Nd 2 O3 NPs的根和叶中的超氧化物歧化酶（SOD）活性（17.5%）和过氧化氢酶（CAT）活性（243.1%）增加。通过定量逆转录聚合酶链反应（RT-PCR）分析，观察到Nd 2 O3纳米颗粒处理的南瓜中SOD和CAT的转录水平上调，证实了这一发现。这些结果表明，TA通过改变颗粒表面电荷来消除Nd 2 O3 BPs/NPs的毒性，从而减少NPs与南瓜的接触和吸收。此外，TA通过提高参与ROS清除的基因的转录水平来促进抗氧化酶活性。我们的研究结果揭示了潜在的机制DOM的影响的生物利用度和毒性的NP陆生植物。
The effect of dissolved organic matter (DOM) on nanoparticle toxicity to plants is poorly understood. In this study, tannic acid (TA) was selected as a DOM surrogate to explore the mechanisms of neodymium oxide NPs (Nd2O3NPs) phytotoxicity to pumpkin (Cucurbita maxima). The results from the tested concentrations showed that 100 mg L−1Nd2O3NPs were significantly toxic to pumpkin in term of fresh biomass, and the similar results from the bulk particles and the ionic treatments were also evident. Exposure to 100 mg L−1of Nd2O3NPs and BPs in 1/5 strength Hoagland’s solution not only significantly inhibited pumpkin growth, but also decreased the S, Ca, K and Mg levels in plant tissues. However, 60 mg L−1TA significantly moderated the observed phytotoxicity, decreased Nd accumulation in the roots, and notably restored S, Ca, K and Mg levels in NPs and BPs treated pumpkin. TA at 60 mg L−1increased superoxide dismutase (SOD) activity in both roots (17.5%) and leaves (42.9%), and catalase (CAT) activity (243.1%) in the roots exposed to Nd2O3NPs. This finding was confirmed by the observed up-regulation of transcript levels of SOD and CAT in Nd2O3NPs treated pumpkin analyzed by quantitative reverse transcription polymerase chain reaction. These results suggest that TA alleviates Nd2O3BPs/NPs toxicity through alteration of the particle surface charge, thus reducing the contact and uptake of NPs by pumpkin. In addition, TA promotes antioxidant enzymatic activity by elevating the transcript levels of genes involved in ROS scavenging. Our results shed light on the mechanisms underlying the influence of DOM on the bioavailability and toxicity of NPs to terrestrial plants.