The persistence of component Theileria parva stocks in cattle immunized with the 'Muguga cocktail' live vaccine against East Coast fever in Uganda
The persistence of component Theileria parva stocks in cattle immunized with the 'Muguga cocktail' live vaccine against East Coast fever in Uganda
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DOI：
10.1017/s003118200400513x
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发表时间：
2004-07-01
期刊：
影响因子：
2.4
通讯作者：
Tait, A
中科院分区：
文献类型：
--
作者：
Oura, CAL;Bishop, R;Tait, A
Muguga鸡尾酒活疫苗包括三种小泰勒虫原种（Muguga、基安布5和水牛来源的塞伦盖蒂转化），已广泛用于东部、中部和南部非洲，其感染和治疗方案旨在保护牛免受东海岸热的影响。我们报告了使用一组多态性微卫星和小卫星标记对三种组分疫苗储备的表征，以及开发了一种区分两种疫苗储备的储备PCR方法。这些标志物，与最近开发的反向线印迹分析的使用，使李解决四个重要的问题，有关疫苗接种。第一，疫苗库存之间的关系有多密切，第二，所有三种库存是否在接种后持续存在并诱导携带状态，第三，是否有证据表明疫苗库存的传播，以及接种疫苗是否可以预防当地基因型的感染？结果表明，Muguga和Serengeti转化原种高度相关，但与基安布5非常不同，后者在接种牛中持续存在，从而建立了携带者状态。没有获得疫苗储备传播给共同放牧动物的证据，尽管这些动物感染了多达8种不同的T。parva基因型显示存在显著水平的蜱攻击。一些接种疫苗的动物感染了局部基因型的子集，为有限的疫苗“突破”提供了证据。
The Muguga cocktail' live vaccine comprises three Theileria parva stocks (Muguga, Kiambu 5 and the buffalo-derived Serengeti-transformed) and has been used extensively in Eastern, Central and Southern Africa with an infection and treatment protocol to protect cattle against East Coast fever. We report the characterization of the three component vaccine stocks using a panel of polymorphic micro-satellite and mini-satellite markers and the development of a stock-derived PCR method that distinguishes two of the vaccine stocks. These markers, with the use of a recently developed Reverse Line Blot assay, have enabled Lis to address four important questions in relation to vaccination. First, how closely related are the vaccine stocks, secondly do all three stocks persist post-vaccination and induce a carrier state, thirdly is there evidence for the transmission of the vaccine stocks and fourthly does vaccination prevent infection with local genotypes ? The results show that Muguga and Serengeti-transformed stocks are highly related but very distinct from Kiambu 5 that persists in vaccinated cattle establishing a carrier state. No evidence was obtained for the transmission of vaccine stocks to co-grazed animals, although these animals were infected with up to 8 different T. parva genotypes showing there was a significant level of tick challenge. Some of the vaccinated animals become infected with a subset of local genotypes providing evidence for limited vaccine 'breakthrough'.