Finding a partner in the ocean: molecular and evolutionary bases of the response to sexual cues in a planktonic diatom.
Finding a partner in the ocean: molecular and evolutionary bases of the response to sexual cues in a planktonic diatom.
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DOI：
10.1111/nph.14557
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发表时间：
2017-07
期刊：
影响因子：
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通讯作者：
Ferrante MI
中科院分区：
文献类型：
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作者：
Basu S;Patil S;Mapleson D;Russo MT;Vitale L;Fevola C;Maumus F;Casotti R;Mock T;Caccamo M;Montresor M;Sanges R;Ferrante MI
微藻作为水生生态系统的初级生产者发挥着重要作用。细胞信号调节它们与环境和其他生物的相互作用，但浮游植物中的这一过程定义不清。使用海洋浮游硅藻多纹拟菱形藻，我们研究了细胞对有性生殖过程中释放的线索的反应，这一事件需要强大的调控机制和对种群动态的影响。我们对P. multistriata的基因组进行了测序，并进行了基因组学和转录组学分析，这使得可以定义基因的获得和丢失，水平基因转移，性别相关基因的保守性和进化率。我们还发现了少量保守的非编码元件。有性生殖影响细胞周期进程，并诱导相反交配类型的不对称反应。G蛋白偶联受体和环磷酸鸟苷（cGMP）参与对性暗示的反应，这总体上需要调节细胞周期，减数分裂相关和营养转运蛋白基因，这表明即使在营养充足的条件下也能精细控制营养摄取。可控的生命周期和P. multistriata的基因组序列允许重建硅藻在其生命周期的关键阶段发生的变化，提供有关其基因进化和推定功能的提示，并为有性生殖研究提供支持。
Microalgae play a major role as primary producers in aquatic ecosystems. Cell signalling regulates their interactions with the environment and other organisms, yet this process in phytoplankton is poorly defined. Using the marine planktonic diatom Pseudo‐nitzschia multistriata, we investigated the cell response to cues released during sexual reproduction, an event that demands strong regulatory mechanisms and impacts on population dynamics. We sequenced the genome of P. multistriata and performed phylogenomic and transcriptomic analyses, which allowed the definition of gene gains and losses, horizontal gene transfers, conservation and evolutionary rate of sex‐related genes. We also identified a small number of conserved noncoding elements. Sexual reproduction impacted on cell cycle progression and induced an asymmetric response of the opposite mating types. G protein‐coupled receptors and cyclic guanosine monophosphate (cGMP) are implicated in the response to sexual cues, which overall entails a modulation of cell cycle, meiosis‐related and nutrient transporter genes, suggesting a fine control of nutrient uptake even under nutrient‐replete conditions. The controllable life cycle and the genome sequence of P. multistriata allow the reconstruction of changes occurring in diatoms in a key phase of their life cycle, providing hints on the evolution and putative function of their genes and empowering studies on sexual reproduction.