Overview of the radiology, histology, and bone morphogenetic protein expression during distraction osteogenesis of the mandible
Overview of the radiology, histology, and bone morphogenetic protein expression during distraction osteogenesis of the mandible
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DOI：
10.2310/7070.2002.34305
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发表时间：
2002-10-01
期刊：
影响因子：
--
通讯作者：
Lessard, ML
中科院分区：
文献类型：
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作者：
Campisi, P;Hamdy, RC;Lessard, ML
前言：牵张成骨(DO)是体内组织工程的一种形式，在此过程中使用截骨术和控制性牵张来延长骨。牵张诱导骨形成的分子信号尚未完全阐明。特别是骨形态发生蛋白(BMPs)在下颌骨DO中的作用尚不清楚。目的：研究下颌骨DO过程中新生骨的放射学和组织学演变特征，并探讨这些变化与BMPs表达的关系。在截骨手术后，将牵张器装置应用于下颌骨的一侧。1周后(潜伏期)，以每12h 0.25 mm的速度开始牵张，持续3周(牵张期)，然后进行3周的固位期。术后每周(1~7周)处死2只动物。切除下颌骨，并对新骨进行放射学和组织学评价。用免疫组织化学方法分析骨形态发生蛋白的表达。结果：有放射学和组织学证据表明牵张期有骨形成。6周时，牵张区内有成熟的编织骨。骨形态发生蛋白2和4在牵张过程中强表达于成骨细胞，在固位过程中强表达于软骨细胞。结论：BMP-7蛋白在下颌骨DO中的表达具有一定的时空分布特征，是下颌骨DO的重要调节因子。了解BMPs的表达有助于使用重组蛋白来提高颅面部DO的成骨速度和质量。
Introduction: Distraction osteogenesis (DO) is a form of in vivo tissue engineering during which an osteotomy and controlled distraction are used to lengthen bone. The molecular signals that govern distraction-induced bone formation have not been fully elucidated. Specifically, the role of bone morphogenetic proteins (BMPs) in DO of the mandible remains unclear.Objective: To characterize the radiologic and histologic evolution of newly formed bone during DO of the mandible and to relate these changes to the expression of BMPs.Methods: Fourteen skeletally mature male rabbits were used. A distractor device was surgically applied to one side of the mandible following osteotomy. After 1 week (latency period), distraction was started at a rate of 0.25 mm every 12 hours for 3 weeks (distraction period) and was followed by a 3-week consolidation period. Two animals were sacrificed each week after surgery (weeks 1 to 7). The mandible was resected and the new bone assessed by radiography and histology. The expression of BMPs was also analyzed using immunohistochemistry.Results: There was radiographic and histologic evidence of bone formation during the distraction period. By week 6, there was mature woven bone within the distraction zone. Bone morphogenetic proteins 2 and 4 were strongly expressed in osteoblasts during distraction and in chondrocytes during consolidation. The expression of BMP-7 was relatively minor.Conclusion: The temporal and spatial pattern of BMP expression suggests that these proteins are important mediators of mandibular DO. Understanding the expression of BMPs may facilitate the use of recombinant proteins to enhance the rate and quality of bone generation during craniofacial DO.