Differential levels of insect herbivory in the field associated with genotypic variation in glucosinolates in Arabidopsis thaliana
Differential levels of insect herbivory in the field associated with genotypic variation in glucosinolates in Arabidopsis thaliana
复制标题
DOI：
10.1007/s10886-008-9498-z
复制
发表时间：
2008-08-01
影响因子：
2.3
通讯作者：
Kliebenstein, Daniel J.
中科院分区：
文献类型：
--
作者：
Bidart-Bouzat, M. Gabriela;Kliebenstein, Daniel J.
硫代葡萄糖苷普遍存在于拟南芥及其十字花科近缘植物中，以其在防御昆虫食草性疾病中的作用而闻名。在一个常见的园艺实验中，我们评估了海桑硫代葡萄糖苷的基因变异，并评估了这种化学与植物损害和适应性之间的关联。具体地说，硫代葡萄糖苷浓度与损伤程度直接相关，与健康程度呈负相关。这些结果与人们的普遍预期相反，即加强化学防御应该会减少昆虫的食草性。由于在这项实地试验中测得的昆虫群落以专业草食动物为主，硫代葡萄糖苷和草食之间的这种正向关系与先前观察到的硫代葡萄糖苷(或其水解产物)吸引专业昆虫的观察结果一致。此外，在这个常见的花园中，硫代葡萄糖苷的多样性似乎影响了食草动物的损害程度。例如，含有烯基硫代葡萄糖苷的基因比含有羟烷基硫代葡萄糖苷的基因有更高的平均损伤水平。结果表明，硫代葡萄糖苷的基因变异可能是决定田间昆虫食草动物利用植物模式的一个主要因素。
Glucosinolates are commonly found in Arabidopsis thaliana and its crucifer relatives, which are known for their role in defense against insect herbivory. In a common garden experiment, we assessed genotypic variation in glucosinolates in A. thaliana and evaluated the association between this chemistry and both plant damage and fitness. Specifically, glucosinolate concentrations were directly associated with damage levels and inversely associated with fitness. These results are contrary to the general expectation that enhanced chemical defense should result in decreased insect herbivory. As the measured insect community in this field trial was dominated by specialist herbivores, this positive relationship between glucosinolates and herbivory agrees with previous observations that glucosinolates (or their hydrolysis products) attract specialist insects. In addition, glucosinolate diversity in this common garden appeared to affect herbivore damage levels. For example, genotypes that contained alkenyl glucosinolates had higher mean damage levels than those that contained hydroxyalkyl glucosinolates. Results suggest that genotypic variation in glucosinolates may be a major factor in determining plant utilization patterns by insect herbivores in the field.