Formate detection by potassium permanganate for enhanced hydrogen production in Escherichia coli
Formate detection by potassium permanganate for enhanced hydrogen production in Escherichia coli
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DOI：
10.1016/j.ijhydene.2008.02.054
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发表时间：
2008-05-01
影响因子：
7.2
通讯作者：
Wood, Thomas K.
中科院分区：
文献类型：
--
作者：
Maeda, Toshinari;Wood, Thomas K.
由于没有高通量筛选，用于产氢的大肠杆菌的诱变是困难的。在这里，我们描述了一种通过高锰酸钾检测甲酸盐来快速检测工程菌产氢增强的方法。大肠杆菌，使用甲酸氢裂解酶系统从甲酸合成氢。(c)2008年国际氢能协会。由爱思唯尔有限公司出版。保留所有权利。
Mutagenesis of Escherichia coli for hydrogen production is difficult since there is no high-throughput screen. Here we describe a method for rapid detection of enhanced hydrogen production by engineered strains by detecting formate via potassium permanganate; in E. coli, hydrogen is synthesized from formate using the formate hydrogen lyase system. (c) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.