Nonomuraea fuscirosea sp. nov., an actinomycete isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch).
Nonomuraea fuscirosea sp. nov., an actinomycete isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch).
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DOI：
10.1099/ijs.0.057877-0
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发表时间：
2014-04
影响因子：
2.8
通讯作者：
Xinhui Zhang;Yuejing Zhang;Junwei Zhao;Chongxi Liu;Shurui Wang;Lingyu Yang;Hairong He;W. Xiang;Xiangjing Wang
中科院分区：
文献类型：
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作者：
Xinhui Zhang;Yuejing Zhang;Junwei Zhao;Chongxi Liu;Shurui Wang;Lingyu Yang;Hairong He;W. Xiang;Xiangjing Wang
从紫茎泽兰根际土壤中分离到一株放线菌，命名为NEAU-dht 8（T）。该生物体具有Nonomuraea属的典型形态学和化学分类学特征。DNA的G+C含量为68.47mol%。根据16 S rRNA基因序列相似性分析，菌株NEAU-dht 8（T）与Maheshkhaliensis 16-5-14（T）的亲缘关系最近。（99.31%），野野村菌GW 14-1925（T）（98.77%），考克斯野野村菌JCM 13931（T）（98.71%），文昌野野村菌210417（T）（98.44%）、Nonomuraea bangladeshensis 5-10-10（T）（98.36%）和Nonomuraea salmonea DSM 43678（T）（98.0%），与Nonomuraea属其它种的相似性均低于98%。基于16 S rRNA基因序列的两种树生成算法表明，该分离物与其最近邻N. maheshkhaliensis 16-5-14（T）.然而，低水平的DNA-DNA相关性允许新分离物与N. maheshkhaliensis 16-5-14（T）.菌株NEAU-dht 8（T）的16 S rRNA基因序列与野野村菌属其它种的相似性较高（98-98.77%）。因此，菌株NEAU-dht 8（T）被认为代表了野野村菌属的一个新种，并建议将其命名为Nonomuraea fuscirosea sp. nov.。模式菌株为NEAU-dht 8（T）（= CGMCC 4.7104（T）= DSM 45880（T））。
A novel actinomycete, designated strain NEAU-dht8(T), was isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Nonomuraea. The G+C content of the DNA was 68.47 mol%. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-dht8(T) was most closely related to Nonomuraea maheshkhaliensis 16-5-14(T) (99.31%), Nonomuraea kuesteri GW 14-1925(T) (98.77%), Nonomuraea coxensis JCM 13931(T) (98.71%), Nonomuraea wenchangensis 210417(T) (98.44 %), Nonomuraea bangladeshensis 5-10-10(T) (98.36%) and Nonomuraea salmonea DSM 43678(T) (98.0%); similarities to other species of the genus Nonomuraea were lower than 98%. Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with its closest neighbour N. maheshkhaliensis 16-5-14(T). However, the low level of DNA-DNA relatedness allowed the novel isolate to be differentiated from N. maheshkhaliensis 16-5-14(T). Strain NEAU-dht8(T) could also be differentiated from other species of the genus Nonomuraea showing high 16S rRNA gene sequence similarity (98-98.77%) by morphological and physiological characteristics. Thus, strain NEAU-dht8(T) is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea fuscirosea sp. nov. is proposed. The type strain is NEAU-dht8(T) ( = CGMCC 4.7104(T) = DSM 45880(T)).