Demonstration of an S1-nuclease sensitive site near the human beta-globin gene, and its protection by HMG 1 and 2.
Demonstration of an S1-nuclease sensitive site near the human beta-globin gene, and its protection by HMG 1 and 2.
复制标题
DOI：
10.1016/0006-291x(83)91499-7
复制
发表时间：
1983-04
影响因子：
3.1
通讯作者：
P. Cockerill;G. Goodwin
中科院分区：
文献类型：
--
作者：
P. Cockerill;G. Goodwin
在含有人β-珠蛋白基因5′侧翼序列的超螺旋质粒中存在S1核酸酶敏感位点。该位点位于基因起始处上游约540个碱基对，在52个交替嘌呤-嘧啶残基的区域内，该区域具有采用十字形结构或Z型DNA的潜力。该位点被高迁移率组染色体蛋白HMG 1和2保护而不被S1核酸酶特异性切割，HMG 1和2可能特异性地起作用以保护单链DNA的短序列。
An S1-nuclease sensitive site exists within supercoiled plasmids containing the 5′-flanking sequences of the human β-globin gene. This site is located approximately 540 base pairs upstream from the start of the gene within a region of 52 alternating purine-pyrimidine residues which has the potential to adopt either cruciform structures or Z-form DNA. This site is protected from specific cleavage by S1-nuclease by the high-mobility-group chromosomal proteins HMG1 and 2, which may be specifically acting to protect short sequences of single-stranded DNA.