Synthesis in E. coli of a polypeptide with human leukocyte interferon activity
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity
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具有人白细胞干扰素活性的多肽在大肠杆菌中的合成
作者：
S. Nagata;H. Taira;A. Hall;L. Johnsrud;M. Streuli;J. Ecsödi;W. Boll;K. Cantell;C. Weissmann
利用pBR322载体，从产生干扰素(IF)的人白细胞中克隆了人外周血白细胞聚(A)RNA的12S片段的双链cDNA。其中一个克隆有一个910个碱基对的插入片段，它可以与IF mRNA杂交，并负责产生具有生物学IF活性的多肽。如果从某些克隆获得每克细胞的活性，则最高可达10,000个单位。
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.