Colonic endocrine cells in inflammatory bowel disease
Colonic endocrine cells in inflammatory bowel disease
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DOI：
10.1046/j.1365-2796.1997.00237.x
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发表时间：
1997-11-01
影响因子：
11.1
通讯作者：
Grimelius, L
中科院分区：
文献类型：
--
作者：
El-Salhy, M;Danielsson, Å;Grimelius, L
目标。研究溃疡性结肠炎 (UC) 和克罗恩病 (CD) 患者的结肠内分泌细胞类型。瑞典于默奥和乌普萨拉大学医院医学和病理学系。科目。 17 名 UC 患者（7 名女性和 10 名男性）和 11 名 CD 患者（5 名女性和 6 名男性）。将 22 名因结肠癌接受手术且没有炎症性肠病迹象的患者（8 名女性和 14 名男性）用作对照。测量。通过免疫组织化学方法鉴定结肠内分泌细胞类型，并通过计算机图像分析进行定量。结果。与对照组相比，UC 和 CD 患者的嗜银细胞面积以及对嗜铬粒蛋白 A 和血清素具有免疫反应的细胞面积显着增加。在CD患者中，多肽YY(PYY)和胰多肽(PP)免疫反应细胞的面积显着减少，而肠高血糖素免疫反应细胞的面积增加。除了 CD 患者的 PYY 和肠高血糖素细胞面积外，轻度炎症标本与重度炎症标本之间的内分泌细胞面积没有统计学差异。在严重炎症的标本中，前者细胞面积减少，而后者细胞面积增加。每种内分泌细胞类型的平均细胞面积在对照组和 UC 或 CD 患者之间没有差异。结论。 UC 和 CD 患者血清素免疫反应细胞面积的增加可能是导致炎症性肠病患者结肠收缩减少和腔内压力增加的因素之一。此外，在 CD 患者中，PYY 免疫反应细胞面积的减少可以解释这些患者中发现的吸收减少和分泌增加的原因。
Objectives. To study colonic endocrine cell types in patients with ulcerative colitis (UC) and Crohn's disease (CD).Setting. Departments of Medicine and Pathology, University Hospitals, Umea and Uppsala, Sweden.Subjects. Seventeen patients with UC (seven females and 10 males) and 11 patients with CD (five females and six males). Twenty-two patients (eight females and 14 males) operated on for colon carcinoma and without signs of inflammatory bowel disease were used as controls.Measurements. The colonic endocrine cell types were identified by immunohistochemical methods and quantified by computed image analysis.Results. The areas of the argyrophil cells as well as these immunoreactive to chromogranin A and serotonin were significantly increased in patients with both UC and CD, compared with those in the controls. In patients with CD, the areas of polypeptide YY(PYY)- and pancreatic polypeptide (PP)-immunoreactive cells were significantly reduced, whilst the area of enteroglucagon-immunoreactive cells was increased. There was no statistical difference in endocrine cell area between specimens with slight versus severe inflammation, except for PYY and enteroglucagon cell areas in patients with CD. Whilst the former cell area decreased, the latter increased in specimens with severe inflammation. The mean cellular area for each endocrine cell type did not differ between the controls and patients with UC or CD.Conclusions. The increase in the serotonin-immunoreactive cell area in patients with both UC and CD might be one of the factors causing reduced colonic contraction and increased intraluminal pressure observed in patients with inflammatory bowel disease. Furthermore, in patients with CD, the decreased PYY-immunoreactive cell area may explain the decreased absorption and increased secretion found in these patients.