Role of serum components in density-dependent inhibition of growth of cells in culture. Platelet-derived growth factor is the major serum determinant of saturation density.
Role of serum components in density-dependent inhibition of growth of cells in culture. Platelet-derived growth factor is the major serum determinant of saturation density.
复制标题
DOI：
10.1083/jcb.85.2.377
复制
发表时间：
1980-05
期刊：
影响因子：
--
通讯作者：
Raines E
中科院分区：
文献类型：
--
作者：
Vogel A;Ross R;Raines E
研究了血小板衍生生长因子和血浆成分对3 T3细胞培养物中饱和密度的影响。这两种成分的全血血清影响饱和密度;然而，当3 T3细胞成为静止在高密度的培养基中含有全血血清，只有血小板衍生的生长因子和新鲜的全血血清能够刺激增殖。加入新鲜血浆来源的血清对细胞生长几乎没有影响。这些结果表明，血小板因子是维持在补充有全血血清的培养基中的3 T3细胞的培养物中的饱和密度的主要决定因素。进行实验以研究血小板衍生生长因子调节饱和密度的机制。我们研究了增殖细胞灭活生长因子的可能性，以及细胞密度对3 T3细胞对血小板衍生生长因子的反应的影响。启动DNA合成所需的血小板衍生生长因子的量随着细胞密度的增加而增加。生长的细胞检测到生长因子的一些失活，但这种消耗仅在高细胞密度下明显。我们提出，培养的3 T3细胞中的密度依赖性抑制是随着培养物变得更加拥挤而对血小板衍生生长因子的需求增加以及生长细胞使生长因子活性失活的结果。
The effects of platelet-derived growth factor and plasma components on saturation density in cultures of 3T3 cells were investigated. Both of these components of whole blood serum affect saturation density; however, when 3T3 cells become quiescent at high density in medium containing whole blood serum, only platelet-derived growth factor and fresh whole blood serum are capable of stimulating proliferation. Addition of fresh plasma- derived serum has little effect on cell growth. These results suggest that the platelet factor is the major determinant of saturation density in cultures of 3T3 cells maintained in medium supplemented with whole blood serum. Experiments were performed to investigate the mechanism by which platelet-derived growth factor regulates saturation density. We investigated the possibilities of inactivation of growth factors by proliferating cells, and the effects of cell density on the response of 3T3 cells to platelet-derived growth factor. The amount of platelet- derived growth factor required to initiated DNA synthesis increases with increasing cell density. Some inactivation of growth factors by growing cells was detected, but this depletion was only evident at high cell density. We propose that density-dependent inhibition in cultured 3T3 cells is the result both of an increased requirement for the platelet- derived growth factor as the cultures become more crowded and of inactivation of growth factor activity by growing cells.