Prevention of SIVmac251 reservoir seeding in rhesus monkeys by early antiretroviral therapy
Prevention of SIVmac251 reservoir seeding in rhesus monkeys by early antiretroviral therapy
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DOI：
10.1038/s41467-018-07881-9
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发表时间：
2018-12-21
影响因子：
16.6
通讯作者：
Barouch, Dan H.
中科院分区：
文献类型：
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作者：
Whitney, James B.;Lim, So-Yon;Barouch, Dan H.
在急性HIV-1感染期间，病毒储存库播种的确切时间尚不清楚。我们先前证明了SIVmac 251感染恒河猴后第3天病毒库被接种。在这里，我们报告了在20只恒河猴（N = 5/组）中直肠内SIVmac 251感染后第0天（6小时）、第1天、第2天或第3天开始ART的影响。在6个月的每日抑制性ART治疗后，停用抗逆转录病毒药物，并监测病毒反弹。在第0天（6 h）、第1天、第2天或第3天开始ART的动物中，分别有0%（0/5）、20%（1/5）、60%（3/5）和100%（5/5）的动物在ART停药后显示病毒反弹，并与淋巴结CD 4 + T细胞中整合的病毒DNA相关。这些数据表明，病毒库在感染的最初几天内接种，早期ART启动限制了病毒库。
The precise time when the viral reservoir is seeded during acute HIV-1 infection remains unclear. We previously demonstrated that the viral reservoir was seeded by day 3 following SIVmac251 infection in rhesus monkeys. Here we report the impact of initiating ART on day 0 (6 h), 1, 2, or 3 following intrarectal SIVmac251 infection in 20 rhesus monkeys (N = 5/group). After 6 months of daily suppressive ART, antiretroviral drugs were discontinued, and viral rebound was monitored. 0% (0 of 5), 20% (1 of 5), 60% (3 of 5), and 100% (5 of 5) of animals that initiated ART on days 0 (6 h), 1, 2, or 3, respectively, showed viral rebound following ART discontinuation and correlated with integrated viral DNA in lymph node CD4+ T cells. These data demonstrate that the viral reservoir is seeded within the first few days of infection and that early ART initiation limits the viral reservoir.