Intranasal administration of CpG DNA lipoplex prevents pulmonary metastasis in mice.
Intranasal administration of CpG DNA lipoplex prevents pulmonary metastasis in mice.
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DOI：
10.1016/j.canlet.2009.05.037
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发表时间：
2010
期刊：
影响因子：
9.7
通讯作者：
Shuwen Zhou;S. Kawakami;F. Yamashita;M. Hashida
中科院分区：
文献类型：
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作者：
Shuwen Zhou;S. Kawakami;F. Yamashita;M. Hashida
合成的含有CpG基序的寡脱氧核苷酸（CpG DNA）可以激活免疫活性细胞，具有抗肿瘤活性的潜在优势。在这项研究中，我们使用阳离子脂质体与CpG DNA （CpG DNA脂质体）配合，以防止小鼠鼻内给药后的肺转移。在colon26/Luc和B16F10细胞攻毒前，经鼻给药CpG DNA脂质体可显著抑制肿瘤细胞的增殖，并延长CpG DNA脂质体小鼠的存活时间。经鼻给药后，[32P] CpG DNA脂质体主要分布于鼻腔和肺部，并诱导肺内IFN-γ产生增加。提示经鼻给药CpG DNA脂质体对小鼠肺转移具有显著的预防作用。
Synthetic oligodeoxynucleotides containing CpG motifs (CpG DNA) can activate immunocompetent cells which offer the potential advantage of antitumor activity. In this study, we used cationic liposomes to complex with CpG DNA (CpG DNA lipoplex) to prevent pulmonary metastasis following intranasal administration in mice. Intranasal administration of CpG DNA lipoplex prior to challenge with both colon26/Luc and B16F10 cells significantly prevented the proliferation of tumor cells, and the survival time of the mice receiving CpG DNA lipoplex was prolonged. After intranasal administration, [32P] CpG DNA lipoplex mainly distributed in nose and lung and induced higher IFN-γ production in the lung. These results suggest that intranasal administration of CpG DNA lipoplex has a significant effect on preventing pulmonary metastasis in mice.