Advanced backcross QTL analysis in barley (Hordeum vulgare L.)
Advanced backcross QTL analysis in barley (Hordeum vulgare L.)
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DOI：
10.1007/s00122-003-1253-9
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发表时间：
2003-07-01
影响因子：
5.4
通讯作者：
Léon, J
中科院分区：
文献类型：
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作者：
Pillen, K;Zacharias, A;Léon, J
本文报道了第一个以春大麦为模型的先进的回交QTL（数量性状位点）计划。BC 2F 2群体来源于初始杂交Apex（Hordeum vulgare ssp. vulgare，下文缩写为Hv）× ISR 101 -23（H. v. ssp. spontaneum，下文缩写为Hsp）。用45个SSR（simple sequence repeat）标记对136个BC_2F_2单株进行了基因分型。随后，收集了136个BC 2F 2家系的田间数据，在最多6个环境中测量了13个数量性状。QTL的检测采用两因子方差分析，标记主效应和标记×环境互作的检测结果均达到P < 0.01的显著水平。在检测的585个标记×性状组合中，共检测到86个QTL。在64个推测QTL上，标记主效应和27个推测QTL上，M × E互作均达到显著水平。在五个案例中，两种影响都很显著。在这些QTL中，有29个（34%）来自外来亲本。在这些标记位点的纯合Hsp基因型与改善的性状相比，纯合Hv基因型。在一种情况下，Hsp等位基因与在六个测试环境中平均7.7%的产量增加相关。在早期的大麦QTL研究中已经报道了同一染色体区域的产量QTL。
This paper reports on the first advanced backcross-QTL (quantitative trait locus) project which utilizes spring barley as a model. A BC2F2 population was derived from the initial cross Apex (Hordeum vulgare ssp. vulgare, hereafter abbreviated with Hv) x ISR101-23 (H. v. ssp. spontaneum, hereafter abbreviated with Hsp). Altogether 136 BC2F2 individuals were genotyped with 45 SSR (simple sequence repeat) markers. Subsequently, field data for 136 BC2F2 families were collected for 13 quantitative traits measured in a maximum of six environments. QTLs were detected by means of a two-factorial ANOVA with a significance level of P < 0.01 for a marker main effect and a marker x environment (M x E) interaction, respectively. Among 585 marker x trait combinations tested, 86 putative QTLs were identified. At 64 putative QTLs, the marker main effect and at 27 putative QTLs, the M x E interaction were significant. In five cases, both effects were significant. Among the putative QTLs, 29 (34%) favorable effects were identified from the exotic parent. At these marker loci the homozygous Hsp genotype was associated with an improvement of the trait compared to the homozygous Hv genotype. In one case, the Hsp allele was associated with a yield increase of 7.7% averaged across the six environments tested. A yield QTL in the same chromosomal region was already reported in earlier barley QTL studies.