Cloning and characterization of ACC oxidase genes from tulip
Cloning and characterization of ACC oxidase genes from tulip
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DOI：
10.5511/plantbiotechnology.24.241
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发表时间：
2007-03
影响因子：
1.6
通讯作者：
K. Momonoi;K. Shoji;Kumi Yoshida
中科院分区：
文献类型：
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作者：
K. Momonoi;K. Shoji;Kumi Yoshida
从郁金香（TulipagesnerianaL.）通过花瓣cDNA文库的差异筛选，将其命名为TgACO 1、TgACO 2、TgACO 3、TgACO 4和TgACO 5。推导的氨基酸序列与其他植物ACO蛋白具有相似性。在这些蛋白质中，TgACO 1 -4彼此具有较高的相似性（90-94%的同一性），而TgACO 5与其他四种蛋白质相比显示出较低的相似性（50%的同一性）。系统发育分析表明，TgACO 1 -4和TgACO 5的亲缘关系较远。TgACO 1 -4的基因组分析表明，它们被组织成四个外显子和三个内含子，而TgACO 5由三个外显子被两个内含子中断。实时RT-PCR分析基因表达显示，TgACO 1，TgACO 3和TgACO 5在枯萎的花瓣，叶，茎，分别表达，而TgACO 2和TgACO 4只在这些组织中的基础水平。因此，郁金香ACO基因似乎在营养组织和花衰老过程中受到差异调节。
Five cDNA clones encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) were isolated from tulip (Tulipa gesneriana L.) by differential screening of the petal cDNA library and were designated as TgACO1, TgACO2, TgACO3, TgACO4 and TgACO5. The deduced amino acid sequences exhibited similarity to ACO proteins from other plant species. Among these proteins, TgACO1-4 have high similarity (90–94% identity) each other, whereas TgACO5 showed low similarity compared with the other four proteins (50% identity). Phylogenic analysis indicated that TgACO1-4 and TgACO5 are distant. Genomic analysis of TgACO1-4 showed that they are organized into four exons and three introns, whereas TgACO5 consists of three exons interrupted by two introns. Real-time RT-PCR analysis of gene expression revealed that TgACO1, TgACO3 and TgACO5 were expressed in wilting petals, leaves, and stems, respectively, whereas TgACO2 and TgACO4 were expressed only at basal levels in these tissues. Therefore, tulip ACO genes seem to be regulated differentially among the vegetative tissues and during flower senescence.