Localization of Porphyromonas gingivalis-carrying fimbriae in situ in human periodontal pockets
Localization of Porphyromonas gingivalis-carrying fimbriae in situ in human periodontal pockets
复制标题
DOI：
10.1177/154405910408301210
复制
发表时间：
2004-12-01
影响因子：
7.6
通讯作者：
Ebisu, S
中科院分区：
文献类型：
--
作者：
Noiri, Y;Li, L;Ebisu, S
菌毛参与黏附，是牙龈卟啉单胞菌的重要致病因素。然而，在体内，牙龈假单胞菌携带菌毛的分布是未知的。原位检测携带牙龈假单胞菌菌毛的定位。从19例重度牙周炎和牙龈卟啉卟啉菌患者中，我们获得了20颗牙周组织附着的牙齿，有或没有免疫定位的菌毛。光镜下11颗牙，电镜下9颗牙。在检查的11个样品中，我们检测到6个抗p阳性反应。牙龈-菌毛血清，位于牙骨质附着斑块的深袋区。在所谓的“无菌斑区”，免疫细胞化学观察到9个样本中有6个携带牙龈假单胞菌的菌毛与牙角质层接触。这些发现表明，携带牙龈假单胞菌的菌毛与人类牙周袋底部的根表面粘附密切相关。
Fimbriae, which are involved in adherence, constitute an important pathogenic factor of Porphyromonas gingivalis. In vivo, however, the distribution of P. gingivalis- carrying fimbriae is unknown. The localization of P. gingivalis-carrying fimbriae was examined in situ. From 19 patients with severe periodontitis and P. gingivalis, we obtained 20 teeth with periodontal tissue attached, with and without immunolocalized fimbriae. Eleven teeth were subjected to light microscopy, 9 to electron microscopy. In 6 of the 11 samples examined, we detected positive reactions with an anti-P. gingivalis- fimbriae serum, located in the cementum-attached plaque area in the deep pocket zones. In the so-called 'plaque-free zones', P. gingivalis- carrying fimbriae were immunocytochemically observed to reside in contact with the dental cuticle in 6 of the 9 samples examined. These findings suggest that P. gingivalis- carrying fimbriae are strongly related to adherence to the root surface at the bottoms of human periodontal pockets.