Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis
Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis
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Tip生长缺陷1与纤维素合酶A3相互作用调节拟南芥中的纤维素生物合成
DOI：
10.1007/s11103-022-01283-8
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发表时间：
2022
影响因子：
5.1
通讯作者：
Youfa Cheng
中科院分区：
文献类型：
--
作者：
Lu Zhang;Madhu Shudan Thapa Magar;Yanning Wang;Youfa Cheng
AtTIP 1与AtCESA 3在物理和遗传上相互作用。AtCESA 3发生S-酰化，可能是由AtTIP 1介导的，这表明AtTIP 1在纤维素生物合成和植物发育中的特定作用。S-酰化是由蛋白质S-酰基转移酶（PATs）催化的蛋白质的可逆的翻译后脂质修饰。S-酰化对于包括纤维素生物合成在内的各种生物分子机制都是重要的。纤维素是由纤维素合成酶A（CESA）复合物（CSC）在质膜上合成的。然而，涉及纤维素生物合成的特定PAT尚未确定，PAT调节CESA的确切机制在很大程度上是未知的。在这里，我们报告的分离tip 1 -5，一个等位基因的尖端生长缺陷1（AtTIP 1/AtPAT 24）与提前终止密码子。tip 1 -5与ixr 1 -2基因相互作用，ixr 1 -2是AtCESA 3的点突变体，其编码CSC合成初生壁纤维素的催化亚基。我们发现AtTIP 1与AtCESA 3物理相互作用。AtCESA 3发生S-酰化，这可能是由AtTIP 1介导的，表明AtTIP 1和AtCESA 3之间的功能关系。与单突变体相比，tip 1 -5 ixr 1 -2双突变体初生花序茎中束间纤维细胞的细胞壁更薄，结晶纤维素含量显著减少。这些结果突出了AtTIP 1在纤维素生物合成中的正调控，以及AtPAT在植物发育中的特定作用。
AtTIP1 physically and genetically interacts with AtCESA3. AtCESA3 undergoes S-acylation, possibly mediated by AtTIP1, suggesting a specific role of AtTIP1 in cellulose biosynthesis and plant development. S-acylation is a reversible post-translational lipid modification of proteins catalyzed by protein S-acyl transferases (PATs). S-acylation is important for various biological molecular mechanisms including cellulose biosynthesis. Cellulose is synthesized by the cellulose synthase A (CESA) complexes (CSCs) at the plasma membrane. However, specific PAT involving in cellulose biosynthesis has not been identified and the precise mechanism by which PAT regulates the CESAs is largely unknown. Here, we report isolation of tip1-5, an allele of Tip Growth Defective1 (AtTIP1/AtPAT24) with a premature stop codon. tip1-5 genetically interacts with ixr1-2, a point mutant of AtCESA3 which encodes a catalytic subunit of CSC synthesizing primary wall cellulose. We show that AtTIP1 physically interacts with AtCESA3. AtCESA3 undergoes S-acylation, which is possibly mediated by AtTIP1, suggesting a functional relationship between AtTIP1 and AtCESA3. Moreover, the interfascicular fiber cells in the primary inflorescence stems of tip1-5 ixr1-2 double mutant contain thinner cell walls and significantly less crystalline cellulose compared to the single mutants. These results highlight the positive regulation of AtTIP1 in cellulose biosynthesis, and a specific role of AtPAT in plant development.
影响因子：
2.9
作者：
Li, Junan;Mahajan, Anjali;Tsai, Ming-Daw
通讯作者：
Tsai, Ming-Daw
影响因子：
9.4
作者：
Hemsley, Piers A.;Weimar, Thilo;Grierson, Claire S.
通讯作者：
Grierson, Claire S.