Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.
Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.
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具有血源性内皮细胞的护理点快速接种心室辅助装置，可形成活的抗血栓涂层。
DOI：
10.1097/mat.0000000000000351
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Achneck,HardeanE
中科院分区：
文献类型：
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作者：
Noviani,Maria;Jamiolkowski,RyanM;Grenet,JustinE;Lin,Qiuyu;Carlon,TimA;Qi,Le;Jantzen,AlexandraE;Milano,CarmeloA;Truskey,GeorgeA;Achneck,HardeanE
心脏移植最有希望的替代品是左心室辅助装置和人工心脏；然而，它们的使用受到血栓并发症的限制。为了减少血栓形成，研究人员开发了烧结钛（Ti）表面，但仍有大约7.5%的患者发生血栓形成。我们发明了一种快速播种技术，通过将烧结钛与人脐带血来源的内皮细胞（hCB-ECs）快速内皮化，将血栓形成的风险降到最低。人类脐带血来源的内皮细胞在几分钟内被播种到烧结钛上，并暴露在易于血栓形成的低流体流动剪切应力下。hCB-ECs在烧结Ti表面粘附形成融合的内皮单层。在快速播种后，将烧结后的Ti暴露在4.4 dynes/ cm2中20小时，导致大约70%的细胞粘附。在流动暴露前，通过体外静态培养快速种子烧结钛，细胞粘附性没有显著提高。此外，粘附的hcb - ec在烧结Ti上仍然具有功能，这可以通过血流诱导的一氧化氮分泌增加和血小板粘附减少来证明。体外静态培养15天后，粘附的hCB-ECs保持代谢活性，表达内皮细胞功能标志物血栓调节蛋白，并降低血小板粘附。总之，我们的研究结果证明了用血源性hCB-ECs快速播种烧结Ti以产生活的抗血栓表面的可行性。
The most promising alternatives to heart transplantation are left ventricular assist devices and artificial hearts; however, their use has been limited by thrombotic complications. To reduce these, sintered titanium (Ti) surfaces were developed, but thrombosis still occurs in approximately 7.5% of patients. We have invented a rapid-seeding technology to minimize the risk of thrombosis by rapid endothelialization of sintered Ti with human cord blood-derived endothelial cells (hCB-ECs). Human cord blood-derived endothelial cells were seeded within minutes onto sintered Ti and exposed to thrombosis-prone low fluid flow shear stresses. The hCB-ECs adhered and formed a confluent endothelial monolayer on sintered Ti. The exposure of sintered Ti to 4.4 dynes/cm 2 for 20 hr immediately after rapid seeding resulted in approximately 70% cell adherence. The cell adherence was not significantly increased by additional ex vivo static culture of rapid-seeded sintered Ti before flow exposure. In addition, adherent hCB-ECs remained functional on sintered Ti, as indicated by flow-induced increase in nitric oxide secretion and reduction in platelet adhesion. After 15 day ex vivo static culture, the adherent hCB-ECs remained metabolically active, expressed endothelial cell functional marker thrombomodulin, and reduced platelet adhesion. In conclusion, our results demonstrate the feasibility of rapid-seeding sintered Ti with blood-derived hCB-ECs to generate a living antithrombotic surface.