TRANSPORT OF LARGE MOLECULES FROM PLASMA TO INTERSTITIAL FLUID AND LYMPH IN DOGS
TRANSPORT OF LARGE MOLECULES FROM PLASMA TO INTERSTITIAL FLUID AND LYMPH IN DOGS
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DOI：
10.1152/ajplegacy.1970.219.6.1595
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发表时间：
1970-01-01
影响因子：
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通讯作者：
RENKIN, EM
中科院分区：
文献类型：
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作者：
GARLICK, DG;RENKIN, EM
方法实验在体重9- 13 kg的杂种狗上进行，用戊巴比妥钠30 mg/kg麻醉。通过腹膜后入路暴露双肾，并在肾血管周围放置长结扎线，用于随后的闭塞。用PE-50管在腘淋巴结远端对两条后腿外侧隐静脉两侧的腘动脉插管。将每条腿的插管引流管（通常为两个）引流至含有草酸钠抗凝剂的去皮玻璃小管中。淋巴流量计算为重量差/收集时间。从颈动脉采集动脉血样。为了促进淋巴液的流动，被动移动的爪子由旋转装置在约100个周期/分钟。在8个实验中，静脉压力在一条腿提高了3045毫米汞柱的大腿周围的袖带充气。静脉压力用水银压力计监测，该水银压力计连接到侧隐静脉分支中的插管上。在五个实验中，通过将一只爪子包裹在绝缘电热带中来加热该爪子。通过热敏电阻继电器将皮肤温度保持在45&0.5 ℃。为了防止过冲，通过加热带的电流被降低到这样的水平，使得循环的接通阶段大于50%。选择温度以诱导血管舒张并促进淋巴液流动，但避免对组织的严重损伤（10，1升）。对测试分子的静脉内输注进行编程以维持示踪剂的恒定动脉浓度。该程序的数据是在初步实验中获得的，在初步实验中，对肾血管闭塞的犬单次静脉注射每种示踪剂，并根据随后的输注实验进行调整。当淋巴液流动稳定时，通常在插管后1小时，取淋巴液和血液的空白样品，并结扎肾结扎线。在时间零点预充-
METHODSGenerul. The experiments were done on mongrel dogs weighing 9-l 3 kg, anesthetized with sodium pentobarbital, 30 mg/kg. Both kidneys were exposed by a retroperitoneal approach and long ligatures were placed around the renal vessels for subsequent occlusion. Popliteal lymphatics on both sides of the lateral saphenous veins in the two hindlegs were cannulated distal to the popliteal lymph node with PE-50 tubing. The cannulated lymphatics(usually two) from each leg drained into small tared glass tubes containing Na oxalate as anticoagulant. Lymph flow was calculated as weight difference/collection time. Arterial blood samples were taken from the carotid artery. To promote lymph flow, the paws were moved passively by a rotary device at approximately 100 cycles/min. In eight experiments, venous pressure in one leg was raised 3045 mm Hg by inflating a cuff around the thigh. Venous pressure was monitored with a mercury manometer attached to a cannula in a branch of the lateral saphenous vein In five experiments one paw was heated by wrapping it in insulated electric heating tape. The skin temperature was maintained at 45 & 0.5 C by a thermistor relay. To prevent overshoot, the current through the heating tape was reduced to such a level that the on phase of the cycle was greater than 50%. The temperature was chosen to induce vasodilatation and promote lymph flow but to avoid serious injury to the tissues (10, 1 l), Intravenous infusions of test molecules were programed to maintain a constant arterial concentration of the tracers. Data for the program were obtained in preliminary experiments in which single intravenous injections of each tracer were given to dogs with occluded renal vessels, and adjustments were made on the basis of subsequent infusion experiments. When lymph flow became steady, usually 1 hr after cannulation, blank samples of lymph and blood were taken and the renal ligatures were tied. At time zero priming in-